对从两名长期护理患者中连续获得的多株耐万古霉素肠球菌分离株进行基因分析。
Genetic analysis of multiple vancomycin-resistant Enterococcus isolates obtained serially from two long-term-care patients.
作者信息
Schoonmaker D J, Bopp L H, Baltch A L, Smith R P, Rafferty M E, George M
机构信息
Wadsworth Center, New York State Department of Health, Albany 12208, USA.
出版信息
J Clin Microbiol. 1998 Jul;36(7):2105-8. doi: 10.1128/JCM.36.7.2105-2108.1998.
Abstract
Fifty-eight vancomycin-resistant enterococcal isolates were obtained from two patients over 9 weeks. Numerous pulsed-field gel electrophoresis fingerprinting types were isolated from each patient. By PCR, all isolates were vanA+. However, many isolates from patient B were found to lack vanA by hybridization. Our results demonstrate the importance of examining multiple isolates, especially from patients who are at high risk of infection.
摘要
在9周内从两名患者身上获取了58株耐万古霉素肠球菌分离株。从每名患者身上分离出了多种脉冲场凝胶电泳指纹图谱类型。通过聚合酶链反应（PCR），所有分离株均为vanA阳性。然而，通过杂交发现患者B的许多分离株缺乏vanA。我们的结果表明检查多个分离株的重要性，特别是对于感染风险高的患者。
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本文引用的文献
1
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Antimicrob Agents Chemother. 1998 Mar;42(3):502-8. doi: 10.1128/AAC.42.3.502.
2
Stability of vancomycin-resistant enterococcal genotypes isolated from long-term-colonized patients.从长期定植患者中分离出的耐万古霉素肠球菌基因型的稳定性
J Infect Dis. 1998 Feb;177(2):378-82. doi: 10.1086/514196.
3
A semiquantitative analysis of the fecal flora of patients with vancomycin-resistant enterococci: colonized patients pose an infection control risk.
Clin Infect Dis. 1997 Oct;25(4):929-30. doi: 10.1086/597643.
4
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Antimicrob Agents Chemother. 1997 Sep;41(9):2016-8. doi: 10.1128/AAC.41.9.2016.
5
Management of infections due to resistant enterococci: a review of therapeutic options.耐万古霉素肠球菌感染的管理：治疗选择综述
J Antimicrob Chemother. 1997 Aug;40(2):161-70. doi: 10.1093/jac/40.2.161.
6
Natural history of colonization with vancomycin-resistant Enterococcus faecium.耐万古霉素屎肠球菌定植的自然史。
Infect Control Hosp Epidemiol. 1995 Dec;16(12):680-5. doi: 10.1086/647041.
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