Gerberding S J, Byers C H
Department of Chemical Engineering, University of Tennessee, Knoxville, USA.
J Chromatogr A. 1998 May 29;808(1-2):141-51. doi: 10.1016/s0021-9673(98)00103-4.
A preparative-scale ion-exchange chromatographic process is described for the separation of the four major proteins and lactose from sweet dairy whey. Experiments using a commercial anion-exchange resin were carried out to determine the optimum conditions for initially separating the proteins alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin, immunoglobulin G and lactose from a sweet dairy whey mixture. The separation was accomplished with simultaneous step elution changes in salt concentration and pH. It was found that the anion-exchange step was most effective in separating beta-lactoglobulin from the feed mixture. Following the anion-exchange separation, its breakthrough curve was processed using a commercial cation-exchange resin to further recover the valuable immunoglobulin G. The whey output from an east Tennessee cheese manufacturer was used as a feedstream for the preparative scale experiments and as a reference in scaling to an economically optimized production level operation.
描述了一种制备规模的离子交换色谱法，用于从甜乳清中分离四种主要蛋白质和乳糖。使用市售阴离子交换树脂进行实验，以确定从甜乳清混合物中初步分离α-乳白蛋白、β-乳球蛋白、牛血清白蛋白、免疫球蛋白G和乳糖的最佳条件。通过同时改变盐浓度和pH值进行分步洗脱来完成分离。发现阴离子交换步骤在从进料混合物中分离β-乳球蛋白方面最有效。阴离子交换分离后，使用市售阳离子交换树脂处理其穿透曲线，以进一步回收有价值的免疫球蛋白G。田纳西州东部一家奶酪制造商的乳清产量用作制备规模实验的进料流，并作为扩大到经济优化生产水平操作的参考。
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