Coskun Ozlem
Department of Biophysics, Canakkale Onsekiz Mart University, Canakkale, Turkey.
North Clin Istanb. 2016 Nov 11;3(2):156-160. doi: 10.14744/nci.2016.32757. eCollection 2016.
Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most common methods of protein purification.
色谱法是一种重要的生物物理技术，可用于分离、鉴定和纯化混合物的成分，以进行定性和定量分析。蛋白质可以根据大小和形状、总电荷、表面存在的疏水基团以及与固定相的结合能力等特性进行纯化。基于分子特征和相互作用类型的四种分离技术利用离子交换、表面吸附、分配和尺寸排阻机制。其他色谱技术基于固定床，包括柱色谱、薄层色谱和纸色谱。柱色谱是蛋白质纯化最常用的方法之一。
