Meng H, Terado T, Kimura H
Department of Experimental Radiology, Shiga University of Medical Science, Otsu, Japan.
Int J Radiat Biol. 1998 May;73(5):503-10. doi: 10.1080/095530098142040.
To investigate apoptosis in murine fibroblasts that are deficient in DNA double-stand breaks (dsb) repair.
BALB/c 3T3 cells and cells from a severe combined immunodeficient (scid) mouse were exposed to X-rays, UV light, bleomycin, etoposide or cisplatin. After exposure, the rate of apoptosis was assayed by propidium iodide staining based on characteristic morphological change.
The scid cells, defective in dsb repair, were extremely sensitive to apoptosis induced by X-rays and bleomycin compared with BALB/c 3T3 cells. The scid cells were slightly sensitive to apoptosis induced by etoposide, an agent producing protein-associated dsb, and to apoptosis induced by agents not causing dsb.
These studies show that sensitivity of cells to apoptosis induced by X-rays or bleomycin is enhanced by the scid mutation and that apoptosis induced by etoposide, which causes protein-associated dsb, is not greatly affected. There may be different processes involved in the induction of apoptosis by X-rays or bleomycin and etoposide.
研究DNA双链断裂（dsb）修复缺陷的小鼠成纤维细胞中的细胞凋亡情况。
将BALB/c 3T3细胞和严重联合免疫缺陷（scid）小鼠的细胞暴露于X射线、紫外线、博来霉素、依托泊苷或顺铂。暴露后，根据特征性形态变化，通过碘化丙啶染色测定细胞凋亡率。
与BALB/c 3T3细胞相比，dsb修复存在缺陷的scid细胞对X射线和博来霉素诱导的细胞凋亡极为敏感。scid细胞对产生蛋白质相关dsb的依托泊苷诱导的细胞凋亡以及对不引起dsb的药物诱导的细胞凋亡稍有敏感。
这些研究表明，scid突变增强了细胞对X射线或博来霉素诱导的细胞凋亡的敏感性，而由产生蛋白质相关dsb的依托泊苷诱导的细胞凋亡则未受到很大影响。X射线或博来霉素与依托泊苷诱导细胞凋亡的过程可能不同。
