Kimura K, Chen D, Lindström E, Yamada H, Zhao C M, Håkanson R
Department of Pharmacology, University of Lund, Sweden.
Regul Pept. 1997 Oct 31;72(2-3):69-77. doi: 10.1016/s0167-0115(97)01027-6.
ECL cells in the oxyntic mucosa secrete histamine and pancreastatin in response to gastrin. The present study examined gastrin-evoked ECL-cell responses over a 10-week time span in terms of individual ECL cells and unit ECL cell volume. Rats were treated with omeprazole (400 micromol/kg per day orally). The concentrations of gastrin and pancreastatin in serum and of histamine and pancreastatin in the oxyntic mucosa were measured as was the activity of the oxyntic mucosal histidine decarboxylase (HDC). The ECL cells were visualized by immunostaining of histamine and examined by electron microscopy. The total ECL cell number and volume, and the mean ECL cell diameter and volume were determined. The HDC, chromogranin A (CGA) and cholecystokinin-B (CCK-B) receptor mRNA concentrations were determined. In terms of individual ECL cells and unit ECL cell volume, the serum pancreastatin concentration, the oxyntic mucosal histamine content, HDC activity, and HDC, CGA and CCK-B receptor mRNA contents increased slowly at first and then leveled off or started to decline after 2 weeks. After 10 weeks all ECL-cell parameters (expressed per unit ECL cell volume) were back to or approaching the starting value. In conclusion, sustained hypergastrinemia first activates each individual ECL cell (with a peak after 1-2 weeks) and then causes gradual functional impairment, the activity returning towards the pre-stimulation level.
胃底黏膜中的肠嗜铬样（ECL）细胞会对胃泌素作出反应，分泌组胺和胰抑制素。本研究在10周的时间跨度内，从单个ECL细胞和单位ECL细胞体积方面，检测了胃泌素诱发的ECL细胞反应。给大鼠口服奥美拉唑（每天400微摩尔/千克）。测定血清中胃泌素和胰抑制素的浓度、胃底黏膜中组胺和胰抑制素的浓度，以及胃底黏膜组氨酸脱羧酶（HDC）的活性。通过组胺免疫染色使ECL细胞可视化，并用电镜进行检查。测定ECL细胞总数和体积，以及平均ECL细胞直径和体积。测定HDC、嗜铬粒蛋白A（CGA）和胆囊收缩素B（CCK - B）受体的mRNA浓度。从单个ECL细胞和单位ECL细胞体积来看，血清胰抑制素浓度、胃底黏膜组胺含量、HDC活性以及HDC、CGA和CCK - B受体mRNA含量起初缓慢增加，2周后趋于平稳或开始下降。10周后，所有ECL细胞参数（以单位ECL细胞体积表示）恢复到或接近起始值。总之，持续性高胃泌素血症首先激活每个单个ECL细胞（1 - 2周后达到峰值），然后导致逐渐的功能损害，活性恢复到刺激前水平。
