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帚尾袋貂(Trichosurus vulpecula)垂体催乳素cDNA的克隆与序列分析。

Cloning and sequence analysis of a pituitary prolactin cDNA from the brushtail possum (Trichosurus vulpecula).

作者信息

Curlewis J D, Saunders M C, Kuang J, Harrison G A, Cooper D W

机构信息

Department of Physiology and Pharmacology, University of Queensland, Australia.

出版信息

Gen Comp Endocrinol. 1998 Jul;111(1):61-7. doi: 10.1006/gcen.1998.7089.

Abstract

Overlapping cDNA partial clones of pituitary prolactin from the marsupial brushtail possum (Trichosurus vulpecula) were isolated and sequenced. The nucleotide and deduced amino acid sequences showed high sequence identity with pig prolactin (84.3 and 92.5%, respectively) and all of the expected structural features of a quadruped prolactin. A prolactin gene tree was constructed and rates of evolution calculated for possum along with several mammalian and nonmammalian prolactins. Possum prolactin was most closely linked to the prolactins of eutherian mammals but branched from the main mammalian line well before the eutherian prolactins. The prolactin/GH family shows variable rates of evolution ranging from 0.3 substitutions/amino acid site/year x 10(9) for pig prolactin to 7.0 substitutions/ amino acid site/year x 10(9) for the mouse. Since divergence from the eutherian mammals, possum prolactin has shown a slow rate of evolution (0.2 substitutions/ amino acid site/year x 10(9)). As expected, the prolactin gene was expressed in the possum pituitary gland but not in the liver, lung, kidney, heart, or mammary gland.

摘要

从有袋动物帚尾袋貂(Trichosurus vulpecula)中分离并测序了垂体催乳素的重叠cDNA部分克隆。核苷酸和推导的氨基酸序列与猪催乳素具有高度的序列同一性(分别为84.3%和92.5%),并且具有四足动物催乳素所有预期的结构特征。构建了催乳素基因树,并计算了袋貂以及几种哺乳动物和非哺乳动物催乳素的进化速率。袋貂催乳素与真兽类哺乳动物的催乳素联系最为紧密,但在真兽类催乳素之前就从主要的哺乳动物谱系中分支出来。催乳素/生长激素家族显示出不同的进化速率,从猪催乳素的0.3个替换/氨基酸位点/年×10^9到小鼠的7.0个替换/氨基酸位点/年×10^9不等。自从与真兽类哺乳动物分化以来,袋貂催乳素显示出缓慢的进化速率(0.2个替换/氨基酸位点/年×10^9)。正如预期的那样,催乳素基因在袋貂的垂体中表达,但在肝脏、肺、肾脏、心脏或乳腺中不表达。

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