Takano H, Ichinose T, Miyabara Y, Yoshikawa T, Sagai M
Research Team for Health Effects of Air Pollutants, National Institute for Environmental Studies, Ibaraki, Japan.
Immunopharmacol Immunotoxicol. 1998 May;20(2):329-36. doi: 10.3109/08923979809038548.
We have previously reported that intratracheal instillation of diesel exhaust particles (DEP) enhances allergen-induced eosinophilic airway inflammation, local expression of interleukin-5 and granulocyte macrophage-colony stimulating factor, and allergen-specific production of IgE and IgG in mice. The present study was undertaken to elucidate the effects of DEP on airway hyperresponsiveness as another characteristic feature of allergic asthma. The animals were randomized into four experimental groups that received intratracheal instillation with vehicle, ovalbumin (OVA), DEP, or the combination of OVA and DEP on a weekly basis for 6 weeks. Respiratory resistance (Rrs) was measured 24 h after the last instillation. An increase in Rrs in animals that inhaled acetylcholine was significantly greater in the combined treatment with OVA and DEP than in the other treatments. The present study indicates that DEP can enhance airway responsiveness associated with allergen exposure, and provides experimental evidence that DEP may deteriorate the pathophysiology of allergen-related respiratory disease such as allergic asthma.
我们之前曾报道，气管内滴注柴油废气颗粒（DEP）可增强变应原诱导的嗜酸性气道炎症、白细胞介素-5和粒细胞巨噬细胞集落刺激因子的局部表达，以及小鼠体内变应原特异性IgE和IgG的产生。本研究旨在阐明DEP对气道高反应性的影响，气道高反应性是过敏性哮喘的另一个特征。将动物随机分为四个实验组，每周一次气管内滴注赋形剂、卵清蛋白（OVA）、DEP或OVA与DEP的组合，持续6周。在最后一次滴注后24小时测量呼吸阻力（Rrs）。吸入乙酰胆碱的动物中，OVA与DEP联合治疗组的Rrs增加显著大于其他治疗组。本研究表明，DEP可增强与变应原暴露相关的气道反应性，并提供实验证据表明DEP可能会使变应原相关的呼吸系统疾病如过敏性哮喘的病理生理学恶化。
