Ichinose Takamichi, Takano Hirohisa, Sadakane Kaori, Yanagisawa Rie, Yoshikawa Toshikazu, Sagai Masaru, Shibamoto Takayuki
Department of Health Sciences, Oita University of Nursing and Health Sciences, 2944-9 Notsuharu, Oita 870-1201, Japan.
J Appl Toxicol. 2004 Jan-Feb;24(1):69-76. doi: 10.1002/jat.949.
Response differences by different strains of mice towards house dust mites (Dermatophagoides farinae) or diesel exhaust particles (DEP) were investigated. Mouse strains BALB/c, ICR and C3H/He received 1 micro g of D. farinae or 1 microg of D. farinae + 50 microg of DEP intratracheally four times at 2-week intervals. Dermatophagoides farinae treatment caused the recruitment of eosinophils and lymphocytes. The order of magnitude of the eosinophilic airway inflammation was BALB/c < ICR < C3H/He mice. The protein levels of eotaxin and IL-5 in lung tissues correlated with the manifestations of eosinophilic airway inflammation by D. farinae administration. Diesel exhaust particles aggravated the manifestation of the eosinophilic inflammation through goblet cell proliferation in the airway and enhanced the local expression of eotaxin and IL-5 in all three strains of mice. The levels of eotaxin and IL-5 in lung tissues corresponded to the pathological changes caused by D. farinae + DEP. The increasing order of production levels of antigen-specific IgG1 by D. farinae or D. farinae + DEP was BALB/c < ICR < C3H/He mice. The significant adjuvant effect of DEP on IgG1 production was observed in the C3H/He mice (P < 0.05). These results suggest that the murine strain differences in the production of eosinophilic airway inflammation by D. farinae + DEP are related to differences in local expression of IL-5 and eotaxin. The enhancing effects of DEP may be mediated by a cytokine increase in the local expression. Antigen-specific IgG1 may be an important immunoglobulin in the pathogenesis of allergic asthma enhanced by DEP.
研究了不同品系小鼠对屋尘螨（粉尘螨）或柴油废气颗粒（DEP）的反应差异。BALB/c、ICR和C3H/He品系小鼠每隔2周经气管内给予1μg粉尘螨或1μg粉尘螨 + 50μg DEP，共4次。粉尘螨处理导致嗜酸性粒细胞和淋巴细胞募集。嗜酸性气道炎症的程度顺序为BALB/c < ICR < C3H/He小鼠。肺组织中嗜酸性粒细胞趋化因子和IL-5的蛋白水平与给予粉尘螨后嗜酸性气道炎症的表现相关。柴油废气颗粒通过气道杯状细胞增殖加重嗜酸性炎症的表现，并增强所有三个品系小鼠中嗜酸性粒细胞趋化因子和IL-5的局部表达。肺组织中嗜酸性粒细胞趋化因子和IL-5的水平与粉尘螨 + DEP引起的病理变化相对应。粉尘螨或粉尘螨 + DEP诱导的抗原特异性IgG1产生水平的递增顺序为BALB/c < ICR < C3H/He小鼠。在C3H/He小鼠中观察到DEP对IgG1产生有显著的佐剂作用（P < 0.05）。这些结果表明，粉尘螨 + DEP诱导的嗜酸性气道炎症产生中的小鼠品系差异与IL-5和嗜酸性粒细胞趋化因子的局部表达差异有关。DEP的增强作用可能由局部表达的细胞因子增加介导。抗原特异性IgG1可能是DEP增强的过敏性哮喘发病机制中的一种重要免疫球蛋白。
