van de Merbel N C, Tinke A P, Oosterhuis B, Jonkman J H, Bohle J F
Pharma-Bio Research International, Zuidlaren, Netherlands.
J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):103-12. doi: 10.1016/s0378-4347(97)00643-9.
A method is described for the determination of pilocarpine and its degradation products isopilocarpine, pilocarpic acid and isopilocarpic acid in human plasma and urine. The method is based on a simple sample preparation step -- ultrafiltration for plasma and dilution for urine samples -- followed by a reversed-phase liquid chromatographic separation of the analytes and detection by means of tandem mass spectrometry. Parameters affecting the performance of these steps are discussed. The high sensitivity and selectivity of the method allow low ng/ml concentrations to be determined for all compounds in plasma and undiluted urine, which enables the investigation of the metabolic fate and elimination of pilocarpine after oral administration to humans.
本文描述了一种测定人血浆和尿液中毛果芸香碱及其降解产物异毛果芸香碱、毛果芸香酸和异毛果芸香酸的方法。该方法基于一个简单的样品制备步骤——血浆超滤和尿液样品稀释——随后通过反相液相色谱分离分析物，并采用串联质谱进行检测。讨论了影响这些步骤性能的参数。该方法的高灵敏度和选择性使得能够测定血浆和未稀释尿液中所有化合物的低ng/ml浓度，从而能够研究毛果芸香碱口服给药后在人体内的代谢命运和消除情况。
