MacDonald S, Smith M E, Bailey C J
Department of Physiology, Medical School, University of Birmingham, UK.
Neurosci Lett. 1998 May 29;248(2):85-8. doi: 10.1016/s0304-3940(98)00312-7.
Immunocytochemistry was used to demonstrate the presence of beta-endorphin, and quantitative autoradiography with [125I]beta-endorphin was used to study beta-endorphin binding sites, in spinal cord of lean and obese diabetic ob/ob mice. The proportion of beta-endorphin-positive neurones was approximately 6-fold higher in the ventral horn, and 2-fold higher in the dorsal horn of ob/ob mice than in lean controls. The maximum density of beta-endorphin binding sites was significantly higher in the dorsal horn and intermediate zone of ob/ob mice. The Kd value for the binding was similar in the ventral horn and intermediate zone in lean and ob/ob mice, but slightly lower in the dorsal horn of ob/ob mice. The findings indicate upregulation of both beta-endorphin and its receptors in spinal neurones of ob/ob mice.
采用免疫细胞化学法来证实β-内啡肽的存在，并利用[125I]β-内啡肽定量放射自显影术研究瘦型和肥胖型糖尿病ob/ob小鼠脊髓中的β-内啡肽结合位点。与瘦型对照小鼠相比，ob/ob小鼠腹角中β-内啡肽阳性神经元的比例高出约6倍，背角中高出2倍。ob/ob小鼠背角和中间带中β-内啡肽结合位点的最大密度显著更高。瘦型和ob/ob小鼠腹角及中间带中结合的Kd值相似，但ob/ob小鼠背角中的Kd值略低。这些发现表明ob/ob小鼠脊髓神经元中β-内啡肽及其受体均上调。
