Ballard L, Yu A, Reiner C, Jonas J
Department of Chemistry, School of Chemical Sciences, Urbana, Illinois, 61801, USA.
J Magn Reson. 1998 Jul;133(1):190-3. doi: 10.1006/jmre.1998.1463.
A novel high-pressure, high-resolution NMR probe is described which operates at a frequency of 500 MHz. The design features an alternative RF coil (8 mm sample tube) for high frequency, sensitivity, probe power, and resolution (< 3.0 x 10(-9)). The probe is capable of pressures to at least 5 kbar over a temperature range of -30 to 80 degreesC, and has a double-tuned 1H/2H circuit which can tune at 1H frequencies of either 300 or 500 MHz. The sensitivity of the 300-MHz circuit is over twice that of previous 10-mm high-pressure NMR probe designs, while at 500 MHz the sensitivity is nearly five times that of previous 300-MHz pressure probes. Potential biochemical applications are demonstrated by 2D NOESY spectra of a Troponin C mutant.
描述了一种新型的高压、高分辨率核磁共振探头，其工作频率为500兆赫。该设计具有用于高频、灵敏度、探头功率和分辨率（<3.0×10⁻⁹）的替代射频线圈（8毫米样品管）。该探头能够在-30至80摄氏度的温度范围内承受至少5千巴的压力，并且具有双调谐¹H/²H电路，可在300或500兆赫的¹H频率下进行调谐。肌钙蛋白C突变体的二维NOESY谱证明了其潜在的生化应用。
