用于制备溶解在低粘度流体中的包封蛋白质以进行核磁共振光谱分析的独立高压单元、装置和方法。
Self contained high pressure cell, apparatus and procedure for the preparation of encapsulated proteins dissolved in low viscosity fluids for NMR spectroscopy.
作者信息
Peterson Ronald W, Wand A Joshua
机构信息
Department of Biochemistry & Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, 19104-6059.
出版信息
Rev Sci Instrum. 2005 Sep;76(9):1-7. doi: 10.1063/1.2038087.
Abstract
The design of a sample cell for high performance nuclear magnetic resonance (NMR) at elevated pressure is described. The cell has been optimized for the study of encapsulated proteins dissolved in low viscosity fluids but is suitable for more general NMR spectroscopy of biomolecules at elevated pressure. The NMR cell is comprised of an alumina toughened zirconia tube mounted on a self-sealing non-magnetic metallic valve. The cell has several advantages including relatively low cost, excellent NMR performance, high pressure tolerance, chemical inertness and a relatively large active volume. Also described is a low volume sample preparation device which allows for the preparation of samples under high hydrostatic pressure and their subsequent transfer to the NMR cell.
摘要
描述了一种用于高压下高性能核磁共振（NMR）的样品池设计。该样品池已针对溶解在低粘度流体中的包封蛋白质的研究进行了优化，但适用于高压下生物分子的更通用NMR光谱分析。NMR样品池由安装在自密封非磁性金属阀上的氧化铝增韧氧化锆管组成。该样品池具有几个优点，包括成本相对较低、NMR性能优异、耐压性高、化学惰性以及相对较大的活性体积。还描述了一种小体积样品制备装置，该装置允许在高静水压力下制备样品并随后将其转移到NMR样品池中。
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