Ladányi A, Nagy J O, Jeney A, Tímár J
Joint Research Organization of the Hungarian Academy of Sciences, Department of Molecular Pathology, Budapest, Hungary.
Pathol Oncol Res. 1998;4(2):108-14. doi: 10.1007/BF02904703.
The effect of a panel of cytokines on the proliferation and type IV collagenase production was studied in four melanoma cell lines of different origin, tumorigenicity and metastatic capacity. TGF-b, TNF-a and to a lesser extent, IL-1a exhibited antiproliferative effect on the cell lines, with some lines showing varying degree of resistance. The sensitivity did not correlate directly with the origin or the biological behavior of the tumor lines, suggesting that cytokine resistance of advanced stage melanoma cells may be relative. IL-2, IL-10 and IL-12 displayed little or no effect on proliferation. The effect of cytokines on metalloproteinase production showed a cell line dependent pattern. Interestingly, those cytokines that exhibited the most pronounced antiproliferative activity, also proved most effective in stimulating collagenase secretion, often simultaneously, in the same line. The results indicate that pleiotropic cytokines can have positive and negative effects simultaneously on various steps of tumor progression.
在四种来源、致瘤性和转移能力不同的黑色素瘤细胞系中，研究了一组细胞因子对细胞增殖和IV型胶原酶产生的影响。转化生长因子-β（TGF-β）、肿瘤坏死因子-α（TNF-α）以及程度较轻的白细胞介素-1α（IL-1α）对这些细胞系表现出抗增殖作用，部分细胞系显示出不同程度的抗性。敏感性与肿瘤细胞系的来源或生物学行为无直接关联，这表明晚期黑色素瘤细胞的细胞因子抗性可能是相对的。白细胞介素-2（IL-2）、白细胞介素-10（IL-10）和白细胞介素-12（IL-12）对增殖几乎没有影响或没有影响。细胞因子对金属蛋白酶产生的影响呈现出细胞系依赖性模式。有趣的是，那些表现出最显著抗增殖活性的细胞因子，在同一细胞系中往往同时也最有效地刺激胶原酶分泌。结果表明，多效性细胞因子可同时对肿瘤进展的各个步骤产生正面和负面影响。
