Newton N E, Ohno K, Abdel-monem M M
J Chromatogr. 1976 Sep 15;124(2):277-85. doi: 10.1016/s0021-9673(00)89743-5.
The 5-dimethylaminonaphthalene-1-sulfonyl (dansyl) derivatives of 1,2-diaminoethane, 1,3-diaminopropane, 1,4-diaminobutane (putrescine), spermidine and spermine have been separated by high-pressure liquid chromatography on a Micropak CN-10 column using a programmed solvent gradient elution. The column eluate is monitored by a fluorescence detector. This method has been used to determine the levles of putrescine, spermidine and spermine in various tissues of rats and in L 1210 leukemic cells of mice grown in culture. The technique is sufficiently sensitive to detect ca. 40pmoles of putrescine and ca. 20 pmoles of spermidine and spermine, is quite specific and can be performed rapidly.
1,2 - 二氨基乙烷、1,3 - 二氨基丙烷、1,4 - 二氨基丁烷（腐胺）、亚精胺和精胺的5 - 二甲基氨基萘 - 1 - 磺酰基（丹磺酰）衍生物，已通过在Micropak CN - 10柱上进行高压液相色谱分离，采用程序溶剂梯度洗脱。柱洗脱液由荧光检测器监测。该方法已用于测定大鼠各种组织以及培养的小鼠L 1210白血病细胞中腐胺、亚精胺和精胺的含量。该技术灵敏度足够高，可检测约40皮摩尔的腐胺和约20皮摩尔的亚精胺和精胺，特异性很强且操作迅速。
