用荧光素衍生试剂高效液相色谱法分离和定量植物组织中的多胺。
Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives.
机构信息
Section of Plant Biology, Cornell University, Ithaca, New York 14853.
出版信息
Plant Physiol. 1985 May;78(1):89-91. doi: 10.1104/pp.78.1.89.
Abstract
High performance liquid chromatography in combination with fluorescence spectrophotometry can be used to separate and quantitate polyamines (putrescine, cadaverine, spermidine, spermine), prepared as their dansyl derivatives, from plant tissue. The procedure gives sensitive and consistent results for polyamine determinations in plant tissue. In a standard mixture, the minimal detection level was less than 1 picomole of polyamines.
摘要
高效液相色谱法结合荧光分光光度法可用于分离和定量多胺（腐胺、尸胺、亚精胺、精胺），方法是将其制备成丹磺酰衍生物。该程序为植物组织中多胺的测定提供了灵敏且一致的结果。在标准混合物中，最低检测水平低于 1 皮摩尔多胺。
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本文引用的文献
1
Analysis of polyamines in higher plants by high performance liquid chromatography.高效液相色谱法分析高等植物中的多胺。
Plant Physiol. 1982 Mar;69(3):701-6. doi: 10.1104/pp.69.3.701.
2
Identification and quantitation of amines by thin-layer chromatography.通过薄层色谱法鉴定和定量胺类物质。
J Chromatogr. 1971 Dec 9;63(1):97-112. doi: 10.1016/s0021-9673(01)85620-x.
3
Separation of the Dns derivatives of polyamines and related compounds by thin-layer and high-pressure liquid chromatography.通过薄层色谱法和高压液相色谱法分离多胺及相关化合物的二硝基苯磺酸盐衍生物
J Chromatogr. 1975 Apr 16;107(2):416-9. doi: 10.1016/0021-9673(75)80022-7.
4
Determination of diamines and polyamines in tissues by high-pressure liquid chromatography.用高压液相色谱法测定组织中的二胺和多胺
J Chromatogr. 1976 Sep 15;124(2):277-85. doi: 10.1016/s0021-9673(00)89743-5.
5
Determination of di-and polyamines by high-performance liquid chromatographic separation of their 5-dimethylaminonaphthalene-1-sulfonyl derivatives.通过高效液相色谱法分离二胺和多胺的5-二甲基氨基萘-1-磺酰基衍生物来测定二胺和多胺。
J Chromatogr. 1978 Jan 1;145(1):29-39. doi: 10.1016/s0378-4347(00)81665-5.
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