Zhang J, Sugahara K, Hashimoto K, Sagara Y, Fontana M, Duprè S, Kodama H
Department of Chemistry, Kochi Medical School, Japan.
Physiol Chem Phys Med NMR. 1997;29(2):199-211.
Human peripheral blood polymorphonuclear leukocytes were preincubated with lanthionine, S-(2-aminoethyl)-L-cysteine, and some of their derivatives found in normal human urine and bovine brain. Among these compounds, lanthionine ketimine and to a lesser extent S-(2-aminoethyl)- L-cysteine ketimine enhanced the N-formyl-methionyl-leucyl-phenylalanine-induced superoxide generation. These ketimines induced tyrosyl phosphorylation of 45 kDa protein of cells. The tyrosyl phosphorylation was markedly increased with time, and the phosphorylation process was dependent on the concentration of both ketimines. However, lanthionine, 1,4-thiomorpholine-3,5-dicarboxylic acid, S-(2-aminoethyl)-L-cysteine and 1,4-thiomorpholine-3-carboxylic acid were without effect both on superoxide generation and on tyrosyl phosphorylation of 45 kDa protein. Lanthionine ketimine and S-(2-aminoethyl)-L-cysteine ketimine also enhanced superoxide generation induced by opsonized zymosan but not the one induced by arachidonic acid and phorbol 12-myristate 13-acetate. Ketimine-primed superoxide generation and tyrosyl phoshorylation of 45 kDa protein were inhibited by genistein, an inhibitor of protein tyrosine kinase, but not by 1-(5-isoquinoline sulfonyl)-2- methylpiperazine, an inhibitor of protein kinase C.
将人外周血多形核白细胞与羊毛硫氨酸、S-(2-氨基乙基)-L-半胱氨酸以及在正常人尿液和牛脑中发现的它们的一些衍生物一起预孵育。在这些化合物中，羊毛硫氨酸酮亚胺以及程度稍小的S-(2-氨基乙基)-L-半胱氨酸酮亚胺增强了N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸诱导的超氧化物生成。这些酮亚胺诱导细胞中45 kDa蛋白的酪氨酸磷酸化。酪氨酸磷酸化随时间显著增加，且磷酸化过程取决于两种酮亚胺的浓度。然而，羊毛硫氨酸、1,4-硫代吗啉-3,5-二羧酸、S-(2-氨基乙基)-L-半胱氨酸和1,4-硫代吗啉-3-羧酸对超氧化物生成和45 kDa蛋白的酪氨酸磷酸化均无影响。羊毛硫氨酸酮亚胺和S-(2-氨基乙基)-L-半胱氨酸酮亚胺也增强了调理酵母聚糖诱导的超氧化物生成，但不增强花生四烯酸和佛波醇12-肉豆蔻酸酯13-乙酸酯诱导的超氧化物生成。蛋白酪氨酸激酶抑制剂染料木黄酮可抑制酮亚胺引发的超氧化物生成和45 kDa蛋白的酪氨酸磷酸化，但蛋白激酶C抑制剂1-(5-异喹啉磺酰基)-2-甲基哌嗪则无此作用。
