Schroeder H, Becker A, Hoellt V
Institute of Pharmacology and Toxicology, Medical Faculty, Otto-von-Guericke University, Magdeburg, Germany.
Exp Brain Res. 1998 Jun;120(4):527-30. doi: 10.1007/s002210050427.
Kindling induced by 13 intraperitoneal injections of 40 mg/kg pentylenetetrazole (PTZ) over a period of 4 weeks resulted in a significant long-lasting increase in both the convulsive susceptibility of animals to the convulsant and the density of the specific [3H]-L-glutamate binding sites in the hippocampus. The quisqualate- and kainate-sensitive [3H]-L-glutamate binding sites were increased 24 h after the final PTZ injection, whereas the N-methyl-D-aspartate (NMDA)-sensitive sites had only a tendency to be enhanced. Furthermore, we investigated [3H]-L-glutamate binding on metabotropic receptors and found a significant increase in the hippocampus following PTZ kindling. In addition, in hippocampal tissue of kindled rats (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD)-stimulated inositol phosphate formation is increased. It can be concluded that the increase in metabotropic glutamate receptor (mGluR) density may be the expression of a specific enhancement in susceptibility of the glutamatergic systems to this excitatory amino acid developing in the course of PTZ-induced kindling.
在4周内通过腹腔注射13次40mg/kg的戊四氮（PTZ）引发点燃效应，导致动物对惊厥剂的惊厥易感性以及海马中特异性[3H]-L-谷氨酸结合位点的密度均出现显著且持久的增加。在最后一次PTZ注射后24小时，对quisqualate和kainate敏感的[3H]-L-谷氨酸结合位点增加，而对N-甲基-D-天冬氨酸（NMDA）敏感的位点仅有增强的趋势。此外，我们研究了代谢型受体上的[3H]-L-谷氨酸结合情况，发现PTZ点燃后海马中的结合显著增加。另外，在点燃大鼠的海马组织中，（±）-1-氨基环戊烷-反式-1,3-二羧酸（反式-ACPD）刺激的肌醇磷酸形成增加。可以得出结论，代谢型谷氨酸受体（mGluR）密度的增加可能是在PTZ诱导的点燃过程中，谷氨酸能系统对这种兴奋性氨基酸敏感性特异性增强的表现。
