Zanassi P, Paolillo M, Schinelli S
Istituto di Farmacologia della Facoltà di Farmacia, Università di Pavia, Italy.
Neurosci Lett. 1998 May 15;247(2-3):83-6. doi: 10.1016/s0304-3940(98)00262-6.
The expression and activity of phospholipase A2 (PLA2) isoforms were investigated in primary cultures of striatal astrocytes. The calcium ionophore A23187 together with the protein kinase C activator phorbol ester was the most potent stimulus in eliciting [3H]arachidonic acid release in the extracellular medium. Reverse transcription coupled to polymerase chain reaction (RT-PCR) showed the presence of the 85 kDa cytosolic PLA2 mRNA and the 14 kDa secretory PLA2 mRNA in untreated astrocytes. Immunoblot experiments with isoform-specific antibodies showed the presence of the cytosolic PLA2 in untreated astrocytes, while the secretory PLA2 was detected only in lipopolysaccharide-treated astrocytes. These data suggest that the two PLA2 isoforms expressed in striatal astrocytes might play different roles in cellular processes mediated by astrocytes.
在纹状体星形胶质细胞的原代培养物中研究了磷脂酶A2（PLA2）同工型的表达和活性。钙离子载体A23187与蛋白激酶C激活剂佛波酯一起是在细胞外培养基中引发[3H]花生四烯酸释放的最有效刺激物。逆转录聚合酶链反应（RT-PCR）显示在未处理的星形胶质细胞中存在85 kDa的胞质型PLA2 mRNA和14 kDa的分泌型PLA2 mRNA。用同工型特异性抗体进行的免疫印迹实验表明，未处理的星形胶质细胞中存在胞质型PLA2，而仅在脂多糖处理的星形胶质细胞中检测到分泌型PLA2。这些数据表明，纹状体星形胶质细胞中表达的两种PLA2同工型可能在星形胶质细胞介导的细胞过程中发挥不同作用。
