Schaub C D, Tankersley C, Schwartz A R, Smith P L, Robotham J L, O'Donnell C P
Department of Anesthesiology, School of Public Health and Hygiene, The Johns Hopkins University, Baltimore, Maryland 21224, USA.
J Appl Physiol (1985). 1998 Jul;85(1):366-71. doi: 10.1152/jappl.1998.85.1.366.
Genetic determinants may contribute to the large variability in arterial blood pressure responses to changes in sleep/wake state in humans. In this study, we developed techniques to examine the relationship between sleep/wake state and mean arterial pressure (MAP) in unrestrained, genetically identical mice (C57BL/6J; n = 9). The left common carotid artery was catheterized, and arterial blood gases were analyzed 24-48 h postsurgery to verify normal respiratory and metabolic function. The animals were then allowed to cycle naturally through sleep/wake states over a 3- to 4-h period while continuous polysomnography and arterial pressure measurements were made. The MAP decreased from quiet wakefulness to non-rapid-eye-movement sleep (9.8 +/- 1.3 mmHg; P < 0.001) and further decreased from non-rapid-eye-movement to rapid-eye-movement sleep (9.7 +/- 1.8 mmHg; P < 0.001). We conclude that the inbred strain of C57BL/6J mice exhibits significant and consistent changes in MAP related to sleep/wake state. Future studies can compare responses in this strain of mice with those in other inbred or transgenic mice to determine whether specific genes regulate arterial blood pressure responses to sleep/wake state.
遗传因素可能导致人类动脉血压对睡眠/觉醒状态变化的反应存在很大差异。在本研究中，我们开发了一些技术来检测自由活动的基因相同小鼠（C57BL/6J；n = 9）的睡眠/觉醒状态与平均动脉压（MAP）之间的关系。将左颈总动脉插入导管，并在术后24 - 48小时分析动脉血气，以验证呼吸和代谢功能正常。然后让动物在3至4小时内自然循环通过睡眠/觉醒状态，同时进行连续多导睡眠图和动脉压测量。MAP从安静觉醒状态降至非快速眼动睡眠时降低（9.8±1.3 mmHg；P < 0.001），从非快速眼动睡眠进一步降至快速眼动睡眠时进一步降低（9.7±1.8 mmHg；P < 0.001）。我们得出结论，C57BL/6J小鼠的近交系在与睡眠/觉醒状态相关的MAP方面表现出显著且一致的变化。未来的研究可以比较该品系小鼠与其他近交或转基因小鼠的反应，以确定是否有特定基因调节动脉血压对睡眠/觉醒状态的反应。
