Klein T, Arias A M
Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
Development. 1998 Aug;125(15):2951-62. doi: 10.1242/dev.125.15.2951.
The Notch signalling pathway plays an important role during the development of the wing primordium, especially of the wing blade and margin. In these processes, the activity of Notch is controlled by the activity of the dorsal specific nuclear protein Apterous, which regulates the expression of the Notch ligand, Serrate, and the Fringe signalling molecule. The other Notch ligand, Delta, also plays a role in the development and patterning of the wing. It has been proposed that Fringe modulates the ability of Serrate and Delta to signal through Notch and thereby restricts Notch signalling to the dorsoventral boundary of the developing wing blade. Here we report the results of experiments aimed at establishing the relationships between Fringe, Serrate and Delta during wing development. We find that Serrate is not required for the initiation of wing development but rather for the expansion and early patterning of the wing primordium. We provide evidence that, at the onset of wing development, Delta is under the control of apterous and might be the Notch ligand in this process. In addition, we find that Fringe function requires Su(H). Our results suggest that Notch signalling during wing development relies on careful balances between positive and dominant negative interactions between Notch ligands, some of which are mediated by Fringe.
Notch信号通路在翅原基发育过程中发挥重要作用，尤其是在翅片和边缘的发育中。在这些过程中，Notch的活性受背侧特异性核蛋白Apterous的活性控制，Apterous调节Notch配体Serrate和边缘信号分子Fringe的表达。另一种Notch配体Delta也在翅的发育和图案形成中发挥作用。有人提出，Fringe调节Serrate和Delta通过Notch发出信号的能力，从而将Notch信号限制在发育中的翅片的背腹边界。在此，我们报告旨在确定翅发育过程中Fringe、Serrate和Delta之间关系的实验结果。我们发现，Serrate不是翅发育起始所必需的，而是翅原基扩展和早期图案形成所必需的。我们提供的证据表明，在翅发育开始时，Delta受apterous控制，可能是此过程中的Notch配体。此外，我们发现Fringe功能需要Su(H)。我们的结果表明，翅发育过程中的Notch信号依赖于Notch配体之间正向和显性负向相互作用的精确平衡，其中一些相互作用由Fringe介导。
