Türkeri L N, Akdaş G, Ozyürek M, Akdaş A
Department of Urology, Ergun Ozalp Research Laboratory, Marmara University, School of Medicine Istanbul, Turkiye.
Arch Esp Urol. 1998 May;51(4):398-404.
To develop an in vitro screening system to predict the response to treatment of common malignancies of the genito-urinary tract.
Flow cytometric analysis and cytotoxicity assays (trypan blue and lactic dehydrogenase colorimetric tests) were performed on hormone resistant prostate cancer cell line PC-3 and primary transitional cell carcinoma samples treated with different antineoplastic agents and their combinations. Apoptosis induced by different agents was also investigated by previously established criteria.
There were 9 bladder tumors (47.4%) in the study group that displayed drug resistance to at least one antineoplastic agent. When the drugs were examined individually, there was resistance to cis-platinum in 3 patients (15.8%), methotrexate in 6 (31.6%), vinblastine in 7 (36.8%), epirubicin in 2 and adriamycin in 2 patients (10.5%). Stratification of patients according to the stage of the tumor revealed statistically significant difference between the superficial and invasive tumors in terms of drug resistance (p < 0.05). In prostate cancer cell line vinblastine treatment resulted in a significant increase in S phase fraction. Percent cytotoxicity by trypan blue exclusion test was 26.1% and was significantly higher than the control group (8.7%, p < 0.002). Also, an increase in apoptotic index after the treatment was observed (44.4% and 12.1%, respectively; p < 0.0001).
Both toxicity assays showed a very good correlation (p < 0.005) and can be used to evaluate the effects of different antineoplastic agents on individual tumors.
开发一种体外筛选系统，以预测泌尿生殖道常见恶性肿瘤的治疗反应。
对激素抵抗性前列腺癌细胞系PC-3和经不同抗肿瘤药物及其组合处理的原发性移行细胞癌样本进行流式细胞术分析和细胞毒性测定（台盼蓝和乳酸脱氢酶比色试验）。还通过先前确立的标准研究了不同药物诱导的凋亡。
研究组中有9例膀胱肿瘤（47.4%）对至少一种抗肿瘤药物表现出耐药性。单独检查药物时，3例患者（15.8%）对顺铂耐药，6例（31.6%）对甲氨蝶呤耐药，7例（36.8%）对长春碱耐药，2例对表柔比星耐药，2例对阿霉素耐药（10.5%）。根据肿瘤分期对患者进行分层显示，浅表性肿瘤和浸润性肿瘤在耐药性方面存在统计学显著差异（p<0.05）。在前列腺癌细胞系中，长春碱处理导致S期比例显著增加。通过台盼蓝排斥试验测得的细胞毒性百分比为26.1%，显著高于对照组（8.7%，p<0.002）。此外，观察到处理后凋亡指数增加（分别为44.4%和12.1%；p<0.0001）。
两种毒性测定显示出非常好的相关性（p<0.005），可用于评估不同抗肿瘤药物对个体肿瘤的作用。
