活性Lyn蛋白酪氨酸激酶在静息血小板的膜微区中选择性富集。
Active Lyn protein tyrosine kinase is selectively enriched within membrane microdomains of resting platelets.
作者信息
Dorahy D J, Burns G F
机构信息
Cancer Research Unit, Faculty of Medicine and Health Sciences, University of Newcastle, Callaghan, 2308 NSW, Australia.
出版信息
Biochem J. 1998 Jul 15;333 ( Pt 2)(Pt 2):373-9. doi: 10.1042/bj3330373.
Abstract
Circulating platelets are primed to respond very rapidly to thrombogenic stimuli, but most platelets complete their lifespan without ever becoming activated. Platelet activation is accompanied by waves of sequential tyrosine phosphorylation thought to involve members of the Src family of protein tyrosine kinases (PTKs). We show here that resting platelets contain highly active pp53/56(Lyn) PTK within membrane microdomains (rafts) isolated biochemically with or without the use of detergent. This fraction is also greatly enriched in the transmembrane glycoprotein CD36, known to associate with Lyn PTK, but in transfection studies we could find no evidence to suggest that CD36 affects the distribution or function of Lyn. Upon platelet activation Lyn activity remains constant or diminishes and pp60(c-src) PTK within this fraction becomes highly activated, indicating the dynamic nature of the membrane microdomains. It is suggested that the function of active Lyn PTK in the resting platelet is to allow prolonged survival of this anucleate cell.
摘要
循环血小板已做好准备，能对血栓形成刺激做出非常快速的反应，但大多数血小板在未被激活的情况下度过其生命周期。血小板激活伴随着一系列酪氨酸磷酸化浪潮，这些磷酸化被认为涉及蛋白酪氨酸激酶（PTK）的Src家族成员。我们在此表明，静息血小板在使用或不使用去污剂进行生化分离的膜微结构域（筏）中含有高活性的pp53/56（Lyn）PTK。该部分还富含跨膜糖蛋白CD36，已知其与Lyn PTK相关，但在转染研究中，我们没有发现证据表明CD36会影响Lyn的分布或功能。血小板激活后，Lyn活性保持恒定或降低，该部分中的pp60（c-src）PTK则变得高度激活，这表明膜微结构域具有动态特性。有人认为，静息血小板中活性Lyn PTK的功能是使这种无核细胞得以长期存活。
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本文引用的文献
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Functional rafts in cell membranes.细胞膜中的功能性筏区
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Translocation of the Csk homologous kinase (Chk/Hyl) controls activity of CD36-anchored Lyn tyrosine kinase in thrombin-stimulated platelets.Csk同源激酶（Chk/Hyl）的易位控制凝血酶刺激的血小板中CD36锚定的Lyn酪氨酸激酶的活性。
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Compartmentalized activation of the high affinity immunoglobulin E receptor within membrane domains.膜结构域内高亲和力免疫球蛋白E受体的区室化激活
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Tyrosine kinase receptors concentrated in caveolae-like domains from neuronal plasma membrane.酪氨酸激酶受体集中于来自神经元质膜的小窝样结构域。
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Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.排除CD45会抑制与富含糖脂的膜结构域相关的p56lck的活性。
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Critical role of Lyn kinase in inhibition of neutrophil apoptosis by granulocyte-macrophage colony-stimulating factor.Lyn激酶在粒细胞-巨噬细胞集落刺激因子抑制中性粒细胞凋亡中的关键作用
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10
Activation of SRC family kinases in human neutrophils. Evidence that p58C-FGR and p53/56LYN redistributed to a Triton X-100-insoluble cytoskeletal fraction, also enriched in the caveolar protein caveolin, display an enhanced kinase activity.人类中性粒细胞中SRC家族激酶的激活。有证据表明，p58C-FGR和p53/56LYN重新分布到Triton X-100不溶性细胞骨架部分，该部分也富含小窝蛋白，显示出增强的激酶活性。
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