莫洛尼鼠白血病病毒中未剪接的含Gag信息高水平表达所需的顺式元件。
cis Elements required for high-level expression of unspliced Gag-containing message in Moloney murine leukemia virus.
作者信息
Oshima M, Odawara T, Hanaki K, Igarashi H, Yoshikura H
机构信息
Department of Bacteriology, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
出版信息
J Virol. 1998 Aug;72(8):6414-20. doi: 10.1128/JVI.72.8.6414-6420.1998.
The 441-nucleotide (nt) region (nt 5325 to 5766) around the splice acceptor (SA) site (nt 5491) was found to be necessary for high-level expression of gag-containing unspliced RNA of Moloney murine leukemia virus (M. Oshima, T. Odawara, T. Matano, H. Sakahira, K. Kuchino, A. Iwamoto, and H. Yoshikura, J. Virol. 70:2286-2295, 1996). Detailed genetic dissection of the 441-nt region revealed that the 5'-end 64 nt (nt 5325 to 5389) were necessary for high-level expression of the unspliced RNA when the spliced RNA was not produced, while the 3'-side 301 nt (nt 5466 to 5766) containing the SA site were necessary for producing spliced RNA. When the spliced RNA was produced, the unspliced RNA could be expressed at a high level even when the 5'-end 64 nt were absent. Probably the virus sequence ensuring the splicing could produce an RNA structure able to compensate for the function of the 5'-end 64-nt region responsible for the expression of the unspliced RNA.
莫洛尼氏鼠白血病病毒（M. Oshima、T. Odawara、T. Matano、H. Sakahira、K. Kuchino、A. Iwamoto和H. Yoshikura，《病毒学杂志》70:2286 - 2295，1996年）含gag的未剪接RNA高水平表达所需的剪接受体（SA）位点（第5491位核苷酸）周围441个核苷酸（nt）区域（nt 5325至5766）被发现。对该441 nt区域的详细基因剖析显示，当未产生剪接RNA时，5'端64 nt（nt 5325至5389）对于未剪接RNA的高水平表达是必需的，而包含SA位点的3'端301 nt（nt 5466至5766）对于产生剪接RNA是必需的。当产生剪接RNA时，即使缺失5'端64 nt，未剪接RNA也能高水平表达。可能确保剪接的病毒序列能够产生一种RNA结构，该结构能够补偿负责未剪接RNA表达的5'端64 nt区域的功能。
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