Bray M, Prasad S, Dubay J W, Hunter E, Jeang K T, Rekosh D, Hammarskjöld M L
Myles H. Thaler Center for AIDS and Human Retrovirus Research, University of Virginia, Charlottesville 22908.
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1256-60. doi: 10.1073/pnas.91.4.1256.
Replication of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev protein. This protein acts in concert with the cis-acting rev-responsive element present in intron-containing RNAs to facilitate nuclear export of these RNAs. Here we show that a cis-acting 219-nucleotide sequence from an unrelated "simple" retrovirus, Mason-Pfizer monkey virus (MPMV), enables Rev-independent HIV-1 replication. This sequence is present in an untranslated region near the 3' end of the MPMV genome. The MPMV element is also able to efficiently substitute for Rev in expression of Gag/Pol and Env proteins from subgenomic constructs. We hypothesize that the MPMV element functions by interacting with a cellular factor that plays a role in mRNA transport analogous to that of the Rev protein. It might be possible to exploit this element in the development of an HIV vaccine.
1型人类免疫缺陷病毒（HIV-1）的复制依赖于病毒Rev蛋白。该蛋白与含内含子RNA中存在的顺式作用Rev反应元件协同作用，以促进这些RNA的核输出。在此我们表明，来自无关“简单”逆转录病毒——猴泡沫病毒（MPMV）的一个219个核苷酸的顺式作用序列能够实现不依赖Rev的HIV-1复制。该序列存在于MPMV基因组3'端附近的一个非翻译区。MPMV元件在亚基因组构建体表达Gag/Pol和Env蛋白时也能够有效替代Rev。我们推测，MPMV元件通过与一种细胞因子相互作用发挥功能，该细胞因子在mRNA转运中发挥的作用类似于Rev蛋白。在开发HIV疫苗时利用这一元件或许是可行的。
