产生含有与抗病毒整合酶单链抗体融合的病毒蛋白R的无感染性1型人类免疫缺陷病毒。
Production of uninfectious human immunodeficiency virus type 1 containing viral protein R fused to a single-chain antibody against viral integrase.
作者信息
Okui N, Kobayashi N, Kitamura Y
机构信息
Division of Molecular Genetics, National Institute of Infectious Diseases, Gakuen, Musashimurayama, Tokyo, Japan.
出版信息
J Virol. 1998 Aug;72(8):6960-4. doi: 10.1128/JVI.72.8.6960-6964.1998.
Abstract
A single-chain antibody (scAb) against human immunodeficiency virus type 1 (HIV-1) integrase was expressed as a fusion protein of scAb and HIV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 293T cells. The expression did not affect virion production much but markedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appears to account for the reduced infectivity.
摘要
一种针对人类免疫缺陷病毒1型（HIV-1）整合酶的单链抗体（scAb）在人293T细胞中作为scAb与HIV-1病毒蛋白R（Vpr）的融合蛋白与HIV-1基因组一起表达。该表达对病毒粒子产生的影响不大，但显著降低了子代病毒粒子的感染性。发现融合蛋白被整合到病毒粒子中。这种整合似乎是感染性降低的原因。
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