Maestri L, Ghittori S, Marraccini P, Zadra P, Imbriani M
Fondazione Salvatore Maugeri I.R.C.C.S. Pavia.
G Ital Med Lav Ergon. 1998 Apr-Jun;20(2):103-6.
A method for routine measurement of urinary trans,trans-muconic acid. A method is proposed which allows the accurate determination of urinary trans,trans-muconic acid (Ma) by HPLC with UV detection. Sample pretreatment consists of a first purification on strong anionic exchange (SAX) cartridges followed by extraction on "end capped" reversed-phase (C18-EC) ones. Purified samples are directly injected onto a chromatographic column (C18, 150 x 4.6 mm I.D., 3 microns) which is eluted with water: methanol: acetic acid (97:2:1, v/v) mixture followed by a "spike" of acetonitrile. The retention time of MA is 15 min, the coefficient of variation is lower than 3%, the limit of detection is 7 micrograms/l. The method seems suitable for accurate biological monitoring of subjects exposed to benzene.
一种常规测定尿中反，反-粘康酸的方法。提出了一种通过高效液相色谱-紫外检测法准确测定尿中反，反-粘康酸（Ma）的方法。样品预处理包括首先在强阴离子交换（SAX）柱上进行纯化，然后在“封端”反相（C18-EC）柱上进行萃取。纯化后的样品直接注入色谱柱（C18，150×4.6mm内径，3微米），用甲醇：水：乙酸（97：2：1，v/v）混合物洗脱，随后加入乙腈“加标”。MA的保留时间为15分钟，变异系数低于3%，检测限为7微克/升。该方法似乎适用于对接触苯的受试者进行准确的生物监测。
