Molica S, Vitelli G, Levato D, Crispino G, Dell'Olio M, Dattilo A, Matera R, Gandolfo G M, Musto P
Divisione Ematologia, Azienda Ospedaliera Pugliese-Ciaccio, Catanzaro, Italy.
Haematologica. 1998 May;83(5):398-402.
CD27, a transmembrane homodimer belonging to the nerve growth factor (NGF) receptor superfamily, is typically expressed on leukemic CD5+ cells in B-cell chronic lymphocytic leukemia (CLL) and found in soluble form in the serum of CLL patients. Therefore, we investigated clinico-biological implications of increased serum levels of sCD27 in an unselected series of B-CLL patients.
Serum CD27 (sCD27) levels were determined at the time of diagnosis in 82 previously untreated B-CLL patients using a sandwich enzyme-linked immunoassay (ELISA). Results were correlated with either clinico-hematological or biological features. Finally, quantitative flow cytometric analyses of membrane CD27 (mCD27) expression were carried out on peripheral blood (PB) cells of 22 B-CLL patients and 5 healthy controls, respectively.
CD27 was found to be expressed on the surface of both resting normal and leukemic B cells. sCD27 levels were significantly higher in B-CLL patients (median value 2150 U/mL) than in healthy controls (median value 220 U/mL) (p < 0.0001). There was a close relationship between sCD27 and soluble TNF-alpha, another molecule belonging to the NGF receptor superfamily. Changes in sCD27 level correlated with clinical stage, beta 2 microglobulin and LDH.
These findings indicate that sCD27 is a reliable marker of tumor mass in B-CLL. Its potential prognostic value should be tested in prospective studies.
CD27是一种跨膜同型二聚体，属于神经生长因子（NGF）受体超家族，通常在B细胞慢性淋巴细胞白血病（CLL）的白血病CD5+细胞上表达，并以可溶性形式存在于CLL患者的血清中。因此，我们在一组未经选择的B-CLL患者中研究了血清sCD27水平升高的临床生物学意义。
采用夹心酶联免疫吸附测定法（ELISA），对82例未经治疗的B-CLL患者在诊断时测定血清CD27（sCD27）水平。结果与临床血液学或生物学特征相关。最后，分别对22例B-CLL患者和5例健康对照者的外周血（PB）细胞进行膜CD27（mCD27）表达的定量流式细胞术分析。
发现CD27在静止的正常B细胞和白血病B细胞表面均有表达。B-CLL患者的sCD27水平（中位数为2150 U/mL）显著高于健康对照者（中位数为220 U/mL）（p < 0.0001）。sCD27与可溶性肿瘤坏死因子-α（另一种属于NGF受体超家族的分子）之间存在密切关系。sCD27水平的变化与临床分期、β2微球蛋白和乳酸脱氢酶相关。
这些发现表明sCD27是B-CLL肿瘤负荷的可靠标志物。其潜在的预后价值应在前瞻性研究中进行检验。
