Friedrich R E, Bartel-Friedrich S, Plambeck K, Schmelzle R
Klinik für Mund-Kiefer-Gesichtschirurgie, Universitäts-Krankenhaus Eppendorf, Universität Hamburg.
Mund Kiefer Gesichtschir. 1998 May;2(3):118-21. doi: 10.1007/s100060050044.
To investigate the distribution of Laminin in experimental microvascular surgery.
Autografts (4 mm) of the left common carotid artery in 20 Wistar rats were harvested after 4 weeks. The specimens were investigated immunohistochemically for the demonstration and distribution of Laminin.
The demonstration and distribution pattern of Laminin was a function of the accuracy of each single suture. In the intima, the organisation and amount of Laminin was directed to the lumen and dependent from the distance and vitality of the vessel segments' cells. The media had in its axis direction the lowest reparative potency to regenerate the continuity break. The adventitia formed a thick cuff around the anastomosis, which was circularly grown through with Laminin-positive vasa vasorum. The marked myointimal hyperplasia in the area around the anastomosis and in the entire autograft was constituted of Laminin-positive extracellular matrix.
Our results support the demand for a careful and atraumatic suture technique in microvascular surgery.
研究层粘连蛋白在实验性微血管手术中的分布情况。
20只Wistar大鼠的左颈总动脉自体移植片（4毫米）在4周后取出。对标本进行免疫组织化学检查，以显示层粘连蛋白并观察其分布。
层粘连蛋白的显示和分布模式取决于每一针缝线的精确程度。在内膜，层粘连蛋白的组织和数量朝向管腔，且取决于血管段细胞的距离和活力。中膜在其轴向方向上修复连续性中断的能力最低。外膜在吻合口周围形成一个厚套，有层粘连蛋白阳性的血管滋养管呈环状穿过。吻合口周围区域及整个自体移植片中明显的肌内膜增生由层粘连蛋白阳性的细胞外基质构成。
我们的结果支持在微血管手术中采用细致且无创的缝合技术的要求。
