Wiseman L L, Hammond W R
J Exp Zool. 1976 Sep;197(3):429-33. doi: 10.1002/jez.1401970316.
Disaggregation of seven-day embryonic chick neural retina tissue by eleven different agents produces cells which require variable periods of time to become aggregation competent in culture. The time required between cell isolation and the beginning of inter-cellular adhesion in rotation culture is dependent upon the particular isolating agent used and ranges from zero to sixty minutes.
用十一种不同的试剂对七日龄鸡胚神经视网膜组织进行分散处理,所产生的细胞在培养中需要不同的时间才能具备聚集能力。从细胞分离到旋转培养中细胞间开始黏附所需的时间取决于所使用的特定分离试剂,范围从零到六十分钟不等。