Dol'nikova A E, Iamskova V P, Sologub A A, Stroeva O G
Ontogenez. 1986 Nov-Dec;17(6):620-7.
A study of aggregation of the retinal cells of 8 and 14 day old chick embryos has revealed two phases in this process. The first phase includes the decrease in the concentration of single cells and the increase in the concentration of aggregates. During the second phase the concentration of aggregates falls at the expense of fusion of smaller aggregates into larger ones. The rate of aggregation at both these phases increases with the initial density of cells and decreases with the age of donor embryos and at a suboptimal temperature of cultivation. Aggregation during the first phase does not depend on the presence in the culture medium of divalent cations and colchicine, the level of protein and RNA synthesis in the cells, whereas aggregation during the second phase depends on all these factors. Comparison of these results with the published data suggests that the retinal cell aggregation during the second phase, unlike the first one, is based on the specific adhesiveness of the cells, which is realized via adhesion molecules resynthesized at the cell surface.
一项对8日龄和14日龄鸡胚视网膜细胞聚集情况的研究揭示了这一过程中的两个阶段。第一阶段包括单细胞浓度的降低和聚集体浓度的增加。在第二阶段，聚集体的浓度下降，代价是较小的聚集体融合成较大的聚集体。这两个阶段的聚集速率都随细胞的初始密度增加而增加，随供体胚胎的年龄增加而降低，并且在培养温度低于最适温度时也会降低。第一阶段的聚集不依赖于培养基中二价阳离子和秋水仙碱的存在、细胞中蛋白质和RNA的合成水平，而第二阶段的聚集则依赖于所有这些因素。将这些结果与已发表的数据进行比较表明，与第一阶段不同，第二阶段的视网膜细胞聚集是基于细胞的特异性黏附性，这种黏附性是通过在细胞表面重新合成的黏附分子实现的。
