关于各种有无血栓形成涂层的金属微线圈血栓形成性的实验研究。

An experimental study on thrombogenicity of various metallic microcoils with or without thrombogenic coatings.

作者信息

Kim T S, Park J H, Lee Y, Chung J W, Han M C

机构信息

Department of Radiology, Seoul National, University College of Medicine, Korea.

出版信息

Invest Radiol. 1998 Jul;33(7):407-10. doi: 10.1097/00004424-199807000-00005.

DOI:10.1097/00004424-199807000-00005

PMID:9659593

Abstract

RATIONALE AND OBJECTIVES

Various metallic microcoils have been used as effective embolic materials (stainless steel, tungsten, and platinum). It also has been reported in several articles that the thrombogenicity of coils increased after soaking in thrombin solutions. To compare the thrombogenicity, the authors performed in vitro study, measuring whole blood clotting time (WBCT) of the various microcoils with and without coating with thrombin, gelatin, or both gelatin and thrombin.

METHODS

Stainless steel, tungsten, and platinum microcoils were used in the study. For thrombin coating, the microcoils were soaked in 4 U/mL thrombin solution for 5 minutes, dried for 30 minutes, then soaked and dried again. For gelatin coating, the microcoils were soaked in 10% gelatin solution for 15 minutes, dried for 30 minutes, then soaked and dried again. For dual coating with both gelatin and thrombin, they were soaked in 10% gelatin solution first for 15 minutes, dried for 45 minutes, and soaked in 4 U/mL thrombin solution for 5 minutes, and dried. The authors introduced 1 mL of fresh blood immediately into the test tube containing the microcoil, and measured the time taken for complete coagulation of the 1 mL blood (WBCT).

RESULTS

Untreated stainless steel, tungsten, and platinum microcoils reduced WBCT from 15.76 minutes to 7.15, 5.64, and 7.27 minutes, respectively. Stainless steel microcoils after soaking in thrombin, gelatin, and both gelatin and thrombin solutions showed WBCT of 5.77, 5.82, and 4.44 minutes, respectively. Tungsten microcoils after soaking in thrombin, gelatin, and both gelatin, and thrombin solutions showed WBCT of 3.25, 4.95, and 3.67 minutes, respectively. Platinum microcoils after soaking in thrombin, gelatin, and both gelatin and thrombin solutions showed WBCT of 5.70, 10.01, and 6.27 minutes, respectively.

CONCLUSIONS

Untreated tungsten microcoils showed superior clot promoting activity compared with untreated stainless steel and platinum microcoils. Clot promoting activity of microcoils was increased after coating with thrombin, or both gelatin and thrombin compared with untreated microcoils.

摘要

原理与目的

多种金属微线圈已被用作有效的栓塞材料（不锈钢、钨和铂）。多篇文章也报道称，微线圈在凝血酶溶液中浸泡后其血栓形成能力增强。为比较血栓形成能力，作者进行了一项体外研究，测量了涂有或未涂有凝血酶、明胶或同时涂有明胶和凝血酶的各种微线圈的全血凝固时间（WBCT）。

方法

本研究使用了不锈钢、钨和铂微线圈。对于凝血酶涂层，将微线圈浸泡在4 U/mL的凝血酶溶液中5分钟，干燥30分钟，然后再次浸泡和干燥。对于明胶涂层，将微线圈浸泡在10%的明胶溶液中15分钟，干燥30分钟，然后再次浸泡和干燥。对于同时涂有明胶和凝血酶的双重涂层，先将它们浸泡在10%的明胶溶液中15分钟，干燥45分钟，再浸泡在4 U/mL的凝血酶溶液中5分钟，然后干燥。作者立即将1 mL新鲜血液引入装有微线圈的试管中，并测量1 mL血液完全凝固所需的时间（WBCT）。

结果

未经处理的不锈钢、钨和铂微线圈分别将WBCT从15.76分钟缩短至7.15、5.64和7.27分钟。浸泡在凝血酶、明胶以及同时浸泡在明胶和凝血酶溶液中的不锈钢微线圈的WBCT分别为5.77、5.82和4.44分钟。浸泡在凝血酶、明胶以及同时浸泡在明胶和凝血酶溶液中的钨微线圈的WBCT分别为3.25、4.95和3.67分钟。浸泡在凝血酶、明胶以及同时浸泡在明胶和凝血酶溶液中的铂微线圈的WBCT分别为5.70、10.01和6.27分钟。