[年轻和老年大鼠心肌的体视学研究]

[Stereology of the myocardium in young and aged rats].

作者信息

Aguila M B, Mandarim-de-Lacerda C A, Apfel M I

机构信息

Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro-UERJ.

出版信息

Arq Bras Cardiol. 1998 Feb;70(2):105-9.

PMID:9659717

Abstract

PURPOSE

To determine the myocardial quantitative changes comparing young and aged animals by using the stereology.

METHODS

Thirty rat hearts were studied (15 rats aged 3 months and 15 other rats aging 15 months). The hearts were removed, weighed, fixed in 10% buffered formaldehyde solution, embedded in paraffin, cut in 7 microns thick slices and stained with HE and picro-sirius stains. In each group, we counted 15 random microscopic fields. The following parameters were studied: Vv (myocyte) and Vv(interstitium)(%) (the volume densities of the cardiac myocyte and interstitium, determined by the point-counting method), and Nv(myocyte) (1/mm3) (the numerical density of the cardiac myocytes, determined with the disector method). The total number of myocytes (N[myocyte]) and the mean volume of the myocytes (V[myocytes]) were also determined. The differences were tested by the Mann-Whitney test.

RESULTS

Cardiac weight increased from 1.1 to 1.7 g, the Vv(myocyte) decreased from 76.7 to 72.2%, the Vv(interstitium) increased from 23.3 to 27.8%. The Nv(myocyte) and the N(myocyte) decreased from 14.76 x 10(4) to 6.19 x 10(4)/mm3 and 15.64 x 10(4) to 10.72 x 10(4) myocytes, respectively. Simultaneously, the V(myocyte) increased from 5.42 x 10(3) to 13.26 x 10(3) mm3. These differences were statistically significant (p < 0.05).

CONCLUSION

Myocardial changes, comparing young rats with aged ones suggest loss of myocytes (increased apoptosis?) with simultaneous myocyte hypertrophy.

摘要

目的

运用体视学方法确定年轻和老年动物心肌的定量变化。

方法

研究了30只大鼠心脏（15只3月龄大鼠和15只15月龄大鼠）。取出心脏，称重，固定于10%缓冲甲醛溶液中，石蜡包埋，切成7微米厚切片，进行苏木精-伊红（HE）染色和苦味酸天狼星染色。每组随机选取15个显微镜视野进行计数。研究以下参数：Vv（心肌细胞）和Vv（间质）（%）（心肌细胞和间质的体积密度，采用点计数法测定），以及Nv（心肌细胞）（1/mm³）（心肌细胞的数密度，采用分割法测定）。还测定了心肌细胞总数（N[心肌细胞]）和心肌细胞平均体积（V[心肌细胞]）。差异采用曼-惠特尼检验。

结果

心脏重量从1.1克增加到1.7克，Vv（心肌细胞）从76.7%降至72.2%，Vv（间质）从23.3%增至27.8%。Nv（心肌细胞）和N（心肌细胞）分别从14.76×10⁴降至6.19×10⁴/mm³和从15.64×10⁴降至10.72×10⁴个心肌细胞。同时，V（心肌细胞）从5.42×10³增至13.26×10³mm³。这些差异具有统计学意义（p<0.05）。