Corbin I, Simcoff R, Novak M, Blackburn B J
Department of Biology, University of Winnipeg, Manitoba, Canada.
Parasitol Res. 1998 Jun;84(6):516-8. doi: 10.1007/s004360050439.
Carbon-13 decoupled 1H spin-echo nuclear magnetic resonance (NMR) spectra, with and without 13C population inversion, of extracts of Taenia crassiceps cysticerci incubated in media containing [3-(13C)]-pyruvate showed 13C enrichment in alanine, lactate, acetate, succinate, and citrate. Labeling in the latter three metabolites provides substantial evidence that the malic enzyme reaction in this cestode also functions in the direction opposite to that in which it is normally portrayed. The direct passage of pyruvate from the cytosol to the mitochondrion is suggested by the greater percentage of 13C detected in acetate relative to succinate.
在含有[3-(13C)]-丙酮酸的培养基中孵育的肥胖带绦虫囊尾蚴提取物的碳-13去耦1H自旋回波核磁共振（NMR）光谱，无论有无13C总体反转，均显示丙氨酸、乳酸、乙酸盐、琥珀酸盐和柠檬酸盐中存在13C富集。后三种代谢物中的标记提供了大量证据，表明这种绦虫中的苹果酸酶反应也以与通常描述相反的方向起作用。相对于琥珀酸盐，在乙酸盐中检测到的13C百分比更高，这表明丙酮酸从细胞质直接进入线粒体。
