Ahrens E T, Laidlaw D H, Readhead C, Brosnan C F, Fraser S E, Jacobs R E
Beckman Institute and Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Magn Reson Med. 1998 Jul;40(1):119-32. doi: 10.1002/mrm.1910400117.
Pathology of fixed spinal cords from transgenic mice with a myelin basic protein (MBP) specific T cell receptor was investigated. These mice spontaneously acquire the demyelinating disease experimental allergic encephalomyelitis (EAE). Several complementary imaging modalities, all on the same tissues, were used to visualize lesions; these included high-field (11.7-T) microscopic diffusion tensor imaging (DTI), T2*-weighted imaging, and optical microscopy on histological sections. Lesions were predominantly in white matter around meninges and vasculature and appeared hyperintense in anatomical images. DTIs showed reduced diffusion anisotropy in the same hyperintense regions, consistent with inflammation and edema. Histology in the same tissues exhibited the characteristic pathology of EAE. Two techniques for visualizing the effective diffusion tensor fields are presented, which display direction, organization, and integrity of neuronal fibers. It is shown that DTI offers intriguing possibilities for visualizing axonal organization and lesions within white matter.
对具有髓鞘碱性蛋白（MBP）特异性T细胞受体的转基因小鼠的固定脊髓病理进行了研究。这些小鼠自发患上脱髓鞘疾病实验性自身免疫性脑脊髓炎（EAE）。使用了几种互补的成像方式，均针对相同组织，以可视化病变；这些方式包括高场（11.7-T）微观扩散张量成像（DTI）、T2*加权成像以及组织学切片上的光学显微镜检查。病变主要位于脑膜和脉管系统周围的白质中，在解剖图像中呈高信号。DTI显示相同高信号区域的扩散各向异性降低，这与炎症和水肿一致。相同组织的组织学表现出EAE的特征性病理。提出了两种可视化有效扩散张量场的技术，它们可显示神经纤维的方向、组织和完整性。结果表明，DTI为可视化白质内的轴突组织和病变提供了有趣的可能性。
