Bertholf R L
Department of Pathology, University of Florida Health Science Center at Jacksonville, USA.
Cell Vis. 1998 Jan-Feb;5(1):67-9.
Technical improvements in the application of molecular biology methods to detection and identification of specific nucleic acid sequences have resulted in more widespread incorporation of these techniques into clinical laboratory services. Gene amplification techniques have been refined and automated, making possible the rapid and economical detection of attomolar and smaller quantities of genetic material. Of particular benefit to clinical chemistry applications is the remarkable specificity of a DNA probe for its complementary sequence. Applications of molecular biology techniques in clinical chemistry include diagnosis of infectious, neoplastic, genetic, and hematological diseases. In addition, the use of oligonucleotides as molecular recognition probes may provide new analytical strategies for a wide range of diagnostic applications that currently rely on antibodies.
将分子生物学方法应用于特定核酸序列的检测和鉴定的技术改进，已使这些技术更广泛地纳入临床实验室服务。基因扩增技术已得到完善和自动化，使得能够快速且经济地检测阿托摩尔及更少量的遗传物质。DNA探针与其互补序列具有显著的特异性，这对临床化学应用特别有益。分子生物学技术在临床化学中的应用包括感染性、肿瘤性、遗传性和血液性疾病的诊断。此外，使用寡核苷酸作为分子识别探针可能为目前依赖抗体的广泛诊断应用提供新的分析策略。
