Qureshi S A, Jackson S P
Wellcome/CRC Institute, Cambridge, United Kingdom.
Mol Cell. 1998 Feb;1(3):389-400. doi: 10.1016/s1097-2765(00)80039-8.
Previous studies have established that Archaea possess a homolog of the eukaryotic basal transcription factor TFIIB, termed TFB, that functions together with the archaeal TATA-binding protein (TBP) to direct transcription by RNA polymerase. Here, we analyze the strong S. shibatae viral (SSV) T6 promoter and show that the region of DNA immediately upstream of the TATA-like A box influences promoter strength. When placed upstream of the much weaker rRNA promoter, this sequence makes it as strong as the T6 promoter. By using a combination of approaches, we show that S. shibatae TFB mediates sequence-specific interactions with DNA flanking the A box. Thus, sequence-specific DNA recognition by TFB and TBP are codeterminants of promoter strength in Archaea.
以往的研究表明,古菌拥有一种真核生物基础转录因子TFIIB的同源物,称为TFB,它与古菌TATA结合蛋白(TBP)共同发挥作用,以指导RNA聚合酶进行转录。在此,我们分析了强嗜热栖热菌病毒(SSV)T6启动子,并表明类TATA A框上游紧邻的DNA区域会影响启动子强度。当将该序列置于弱得多的rRNA启动子上游时,它会使其与T6启动子一样强。通过使用多种方法相结合,我们表明嗜热栖热菌TFB介导与A框侧翼DNA的序列特异性相互作用。因此,TFB和TBP对DNA的序列特异性识别是古菌启动子强度的共同决定因素。