多聚血红蛋白-超氧化物歧化酶-过氧化氢酶作为一种具有抗氧化特性的血液替代品。
Polyhemoglobin-superoxide dismutase-catalase as a blood substitute with antioxidant properties.
作者信息
D'Agnillo F, Chang T M
机构信息
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Canada.
出版信息
Nat Biotechnol. 1998 Jul;16(7):667-71. doi: 10.1038/nbt0798-667.
Polyhemoglobin-superoxide dismutase-catalase is designed to function as an oxygen carrier with antioxidant properties. This is based on cross-linking hemoglobin with superoxide dismutase and catalase (PolyHb-SOD-CAT). This study describes the structural and antioxidant properties of this solution. Our studies show that superoxide dismutase and catalase retain their enzymatic activity following glutaraldehyde polymerization with 8:1 and 16:1 glutaraldehyde:hemoglobin ratio. We have analyzed the optimal SOD/CAT ratios to prevent oxidation of hemoglobin in the presence of oxygen free radicals. The circulation half-life of crosslinked hemoglobin, SOD, and catalase in Sprague-Dawley rats correlates with the degree of polymerization as determined by high-performance molecular weight gel filtration. PolyHb-SOD-CAT decreases the formation of oxygen radicals compared with PolyHb in a rat intestinal ischemia-reperfusion model.
聚血红蛋白-超氧化物歧化酶-过氧化氢酶被设计成具有抗氧化特性的氧载体。这是基于将血红蛋白与超氧化物歧化酶和过氧化氢酶交联（聚血红蛋白-超氧化物歧化酶-过氧化氢酶）。本研究描述了该溶液的结构和抗氧化特性。我们的研究表明，在戊二醛与血红蛋白比例为8:1和16:1的情况下进行戊二醛聚合后，超氧化物歧化酶和过氧化氢酶仍保留其酶活性。我们分析了在存在氧自由基的情况下防止血红蛋白氧化的最佳超氧化物歧化酶/过氧化氢酶比例。通过高效分子量凝胶过滤测定，交联血红蛋白、超氧化物歧化酶和过氧化氢酶在斯普拉格-道利大鼠体内的循环半衰期与聚合程度相关。在大鼠肠缺血再灌注模型中，与聚血红蛋白相比，聚血红蛋白-超氧化物歧化酶-过氧化氢酶减少了氧自由基的形成。
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