多聚血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶：一种基于生物技术的新型血液替代品，可运输氧气和二氧化碳，并具有抗氧化作用。
Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase: a novel biotechnology-based blood substitute that transports both oxygen and carbon dioxide and also acts as an antioxidant.
作者信息
Bian Yuzhu, Rong Zhixia, Chang Thomas Ming Swi
机构信息
Artificial Cells and Organs Research Centre, Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):127-36. doi: 10.3109/10731199.2011.581052.
Abstract
Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA) is a therapeutic antioxidant that also transports both oxygen and carbon dioxide. This is formed by crosslinking Hb with SOD, CAT, and CA using glutaraldehyde. Crosslinking stroma free Hb from red blood cell (rbc) reduces CA activity to 55%. Addition of more CA resulted in a preparation with the same CA activity as RBC. PolyHb in the complex acts as a buffer to prevent large pH changes as carbon dioxide is converted to carbonic acid. We then prepare and optimize a novel PolyHb-SOD-CAT-CA, a therapeutic antioxidant that also transports both oxygen and carbon dioxide.
摘要
多聚血红蛋白-超氧化物歧化酶-过氧化氢酶-碳酸酐酶（PolyHb-SOD-CAT-CA）是一种兼具治疗作用的抗氧化剂，同时还能运输氧气和二氧化碳。它是通过戊二醛将血红蛋白与超氧化物歧化酶、过氧化氢酶和碳酸酐酶交联而成的。将红细胞（rbc）中的无基质血红蛋白进行交联会使碳酸酐酶活性降低至55%。添加更多的碳酸酐酶可得到一种碳酸酐酶活性与红细胞相同的制剂。复合物中的多聚血红蛋白起到缓冲作用，防止二氧化碳转化为碳酸时pH值发生大幅变化。然后，我们制备并优化了一种新型的PolyHb-SOD-CAT-CA，它是一种兼具治疗作用的抗氧化剂，同时还能运输氧气和二氧化碳。
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