更昔洛韦在人视网膜中的免疫组织化学定位。

Immunohistochemical localization of ganciclovir in the human retina.

作者信息

Park S S, Girard B, Font R L, Hauw J J, Young L H

机构信息

Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057, USA.

出版信息

Curr Eye Res. 1998 Jun;17(6):663-7.

PMID:9663857

Abstract

PURPOSE

To localize ganciclovir in the retina of human eyes treated with intravenous or intravitreal ganciclovir for cytomegalovirus (CMV) retinitis.

METHODS

Paraffin-embedded five-micron sections of autopsy eyes were obtained from seven patients as follows: two patients with CMV retinitis treated with intravenous ganciclovir; two patients with CMV retinitis treated with an intravitreal sustained-release ganciclovir device; one patient with CMV retinitis treated with intravenous foscarnet; and two patients with AIDS without CMV retinitis who did not receive any anti-CMV therapy. The paraffin was removed from the sections, and indirect immunofluorescent staining was performed, using an antiserum to ganciclovir.

RESULTS

Bright fluorescent staining was noted in the retinal pigment epithelium (RPE) and photoreceptor outer segments of eyes treated with intravenous or intravitreal ganciclovir, but not in eyes treated with foscarnet or without CMV retinitis. In addition, patches of bright fluorescent staining of the internal limiting membrane was noted in eyes treated with intravitreal ganciclovir.

CONCLUSIONS

Ganciclovir is detected in the outer retina of patients with CMV retinitis treated with intravenous or intravitreal therapy. The drug is detected also in the internal limiting membrane in eyes treated with the intravitreal sustained-release ganciclovir device.

摘要

目的

将更昔洛韦定位在接受静脉注射或玻璃体内注射更昔洛韦治疗巨细胞病毒（CMV）视网膜炎的人眼视网膜中。

方法

从七名患者的尸检眼获取石蜡包埋的5微米切片，如下所示：两名接受静脉注射更昔洛韦治疗的CMV视网膜炎患者；两名接受玻璃体内缓释更昔洛韦装置治疗的CMV视网膜炎患者；一名接受静脉注射膦甲酸治疗的CMV视网膜炎患者；以及两名未接受任何抗CMV治疗且无CMV视网膜炎的艾滋病患者。从切片中去除石蜡，使用抗更昔洛韦抗血清进行间接免疫荧光染色。