Di Felice V, Lauricella M, Giuliano M, Emanuele S, Vento R, Tesoriere G
Institute of Biological Chemistry, University of Palermo, Palermo, Italy.
Int J Oncol. 1998 Aug;13(2):225-32. doi: 10.3892/ijo.13.2.225.
This study demonstrated that cisplatin and carboplatin stimulate apoptosis in human retinoblastoma Y79 cells, cisplatin being the most effective compound. The apoptotic effect appeared after 8 h and then increased in a time-dependent manner. Treatment with cisplatin and carboplatin also provoked an increase in the level of p53 and p21, and a lowering in Bcl-2. The prolonged exposure of Y79 cells to cisplatin induced resistance to cisplatin, carboplatin and etoposide. The basal level of p53 was in resistant cells higher than in untreated cells, while Bcl-2 was not modified. p53 and Bcl-2 levels did not change after treating of resistant cells with cisplatin, carboplatin or etoposide. However, camptothecin which is a powerful inducer of apoptosis in sensitive cells, triggered cell death even in resistant cells. Such an effect was not accompanied by any modification in p53 level while Bcl-2 was markedly reduced.
本研究表明，顺铂和卡铂可刺激人视网膜母细胞瘤Y79细胞凋亡，其中顺铂是最有效的化合物。凋亡效应在8小时后出现，然后呈时间依赖性增加。顺铂和卡铂处理还导致p53和p21水平升高，Bcl-2水平降低。Y79细胞长期暴露于顺铂会诱导对顺铂、卡铂和依托泊苷的耐药性。耐药细胞中p53的基础水平高于未处理细胞，而Bcl-2未发生改变。用顺铂、卡铂或依托泊苷处理耐药细胞后，p53和Bcl-2水平没有变化。然而，喜树碱作为敏感细胞中强大的凋亡诱导剂，即使在耐药细胞中也能引发细胞死亡。这种效应并未伴随着p53水平的任何改变，而Bcl-2则明显降低。
