One-tube RT-PCR with sequence-specific primers.
作者信息
Pfeffer U
机构信息
Laboratory of Molecular Biology, National Institute of Genoa, Italy.
出版信息
Methods Mol Biol. 1998;86:143-51. doi: 10.1385/0-89603-494-1:143.
PMID:9664465
Abstract
摘要
相似文献
1
One-tube RT-PCR with sequence-specific primers.使用序列特异性引物的单管逆转录聚合酶链反应。
Methods Mol Biol. 1998;86:143-51. doi: 10.1385/0-89603-494-1:143.
2
Quantitative analysis of RNA species by PCR and solid-phase minisequencing.通过聚合酶链式反应（PCR）和固相微测序对RNA种类进行定量分析。
Methods Mol Biol. 1998;86:121-31. doi: 10.1385/0-89603-494-1:121.
3
Efficient one-tube RT-PCR amplification of rare transcripts using short sequence-specific reverse transcription primers.
Biotechniques. 1995 Feb;18(2):204-6.
4
Gene expression analysis using quantitative reverse transcription-polymerase chain reaction and a multispecific internal control.使用定量逆转录-聚合酶链反应和多特异性内参进行基因表达分析。
Methods Mol Biol. 2000;137:59-73. doi: 10.1385/1-59259-066-7:59.
5
Inverse PCR. cDNA cloning.反向聚合酶链反应。互补脱氧核糖核酸克隆。
Methods Mol Biol. 2002;192:293-9. doi: 10.1385/1-59259-177-9:293.
6
Subtractive cloning of differential genes using RNA-PCR.利用RNA-PCR技术进行差异基因的消减克隆
Methods Mol Biol. 2003;221:253-9. doi: 10.1385/1-59259-359-3:253.
7
Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads.基于长距离PCR和磁珠的多轮消减杂交直接克隆全长细胞差异表达基因
Methods Mol Biol. 2002;192:99-108. doi: 10.1385/1-59259-177-9:099.
8
Subtraction hybridization cDNA libraries.消减杂交cDNA文库
Methods Mol Biol. 1995;37:13-30. doi: 10.1385/0-89603-288-4:13.
9
Improved blood RNA extraction microtechnique for RT-PCR.
Biotechniques. 1995 May;18(5):788-90, 792.
10
Characterization of RNA using continuous RT-PCR coupled with ELOSA.使用连续逆转录聚合酶链反应结合酶联免疫吸附测定法对RNA进行表征。
Methods Mol Biol. 1998;86:161-72. doi: 10.1385/0-89603-494-1:161.
Zotero 插件