Bauer C K, Engeland B, Wulfsen I, Ludwig J, Pongs O, Schwarz J R
Physiologisches Instiut, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
Recept Channels. 1998;6(1):19-29.
The rat homologue of the human ether-ä-go-go-related gene (r-erg) was cloned from rat brain using homology screening. RERG has a 96% amino acid identify to HERG. Membrane currents recorded in CHO cells after previous injection of r-erg showed that the voltage- and time-dependent properties are indistinguishable from h-erg-induced currents expressed in the same system. RT-PCR revealed the presence of r-erg mRNA in clonal rat pituitary cells (GH3/B6 cells). These cells exhibit a voltage-dependent inward-rectifying K current (IK, IR) which is highly sensitive to the class III antiarrhythmic E-4031. IK, IR recorded in GH3/B6 cells and ERG currents in CHO cells were compared using similar experimental conditions (same pulse protocols and isotonic KCl as extracellular solution). The voltage- and time-dependent properties of both currents were found to be almost identical. These results strongly suggest that RERG channels mediate IK, IR in GH3/B6 cells.
利用同源性筛选从大鼠脑内克隆出了人类醚 - 去极化相关基因（r - erg）的大鼠同源物。RERG与HERG有96%的氨基酸同一性。先前向CHO细胞中注射r - erg后记录到的膜电流显示，其电压依赖性和时间依赖性特性与在同一系统中表达的h - erg诱导电流无法区分。RT - PCR揭示了克隆的大鼠垂体细胞（GH3/B6细胞）中存在r - erg mRNA。这些细胞表现出一种对III类抗心律失常药E - 4031高度敏感的电压依赖性内向整流钾电流（IK, IR）。在相似的实验条件下（相同的脉冲方案和等渗KCl作为细胞外溶液），比较了GH3/B6细胞中记录到的IK, IR和CHO细胞中的ERG电流。发现这两种电流的电压依赖性和时间依赖性特性几乎相同。这些结果强烈表明RERG通道介导了GH3/B6细胞中的IK, IR。
