Stevenson R J, Hamilton S J, MacCallum D E, Hall P A, Fuller-Pace F V
Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Medical School, U.K.
J Pathol. 1998 Apr;184(4):351-9. doi: 10.1002/(SICI)1096-9896(199804)184:4<351::AID-PATH1235>3.0.CO;2-C.
The human DEAD box protein p68 is an established RNA-dependent ATPase and RNA helicase, p68 has been highly conserved in evolution and appears to be essential for normal growth, suggesting that this protein plays an important role in the cell. Although the biochemical activities of p68 are fairly well characterized, little is known about its biological function. This report shows that p68 is detectable in quiescent cell lines, but its expression is induced by serum, suggesting that this protein may play a role in cell growth. It is also shown that both p68 mRNA and protein are differentially expressed in adult tissues; in this case, however, the levels do not always correlate with proliferation status, suggesting that the regulation of expression in the animal may be different from that in cell lines. Finally, it is shown that p68 expression is developmentally regulated and appears to correlate with organ differentiation/maturation. These findings suggest that p68 expression may not simply reflect proliferation/differentiation status and that it appears to be regulated in a more complex way.
人类DEAD盒蛋白p68是一种公认的依赖RNA的ATP酶和RNA解旋酶，p68在进化过程中高度保守，似乎对正常生长至关重要，这表明该蛋白在细胞中发挥着重要作用。尽管p68的生化活性已得到相当充分的表征，但其生物学功能却鲜为人知。本报告表明，在静止细胞系中可检测到p68，但其表达受血清诱导，这表明该蛋白可能在细胞生长中发挥作用。研究还表明，p68 mRNA和蛋白在成年组织中存在差异表达；然而，在这种情况下，其水平并不总是与增殖状态相关，这表明该蛋白在动物体内的表达调控可能与在细胞系中不同。最后，研究表明p68的表达受发育调控，且似乎与器官分化/成熟相关。这些发现表明，p68的表达可能不仅仅反映增殖/分化状态，而且其调控方式似乎更为复杂。
