Sanada O, Fukuda Y, Sumimoto R, Hoshino S, Nishihara M, Kaneda K, Asahara T, Dohi K
Second Department of Surgery, School of Medicine, Hiroshima University, Japan.
Transpl Int. 1998;11 Suppl 1:S174-8. doi: 10.1007/s001470050455.
In this study, we investigated whether establishment of chimerism in donor liver with recipient-type bone marrow cells (BMCs) prior to liver transplantation could prolong the liver allograft survival. Donor female ACI rats were inoculated with recipient-type BMCs of male LEW rats via the portal vein, with or without irradiation as cytoablation, followed by intramuscular administration of FK506 for 5 days. At 1-2 months later, livers were harvested and transplanted into naive female LEW rats. No immunosuppressants were used. Chimerism in donor rats was confirmed by primers specific for the sex determinant Y chromosome of rats. With livers from rats pretreated with recipient-type BMCs, survival of liver allografts was significantly extended, irrespective of irradiation. These results showed that modification of the donor liver by intraportal injection of recipient-type BMCs and concomitant administration of FK506 prior to liver transplantation prolonged liver allograft survival in rats.
在本研究中，我们调查了肝移植前用受体型骨髓细胞（BMC）在供体肝脏中建立嵌合体是否能延长肝移植存活时间。将雄性LEW大鼠的受体型BMC经门静脉接种到雌性供体ACI大鼠体内，进行或不进行照射作为细胞消融，随后肌肉注射FK506 5天。1至2个月后，收获肝脏并移植到未经处理的雌性LEW大鼠体内。未使用免疫抑制剂。通过大鼠性别决定Y染色体特异性引物证实供体大鼠存在嵌合体。使用经受体型BMC预处理的大鼠肝脏，无论是否进行照射，肝移植存活时间均显著延长。这些结果表明，肝移植前通过门静脉注射受体型BMC并同时给予FK506对供体肝脏进行处理可延长大鼠肝移植的存活时间。
