Gagnon J, Lago F, Chagnon Y C, Pérusse L, Näslund I, Lissner L, Sjöström L, Bouchard C
Physical Activity Sciences Laboratory, Laval University, Ste-Foy, Quebec, Canada.
Int J Obes Relat Metab Disord. 1998 Jun;22(6):500-5. doi: 10.1038/sj.ijo.0800613.
To investigate the relationships between the A-G point mutation at position -3826 bp in the 5' flanking domain of the uncoupling protein 1 (UCP1 A-3826G) and some obesity phenotypes in the Swedish Obese Subjects (SOS) cohorts of obese and non-obese men and women. Previous studies have supported the hypothesis of an association between the UCP1 A-3826G polymorphism and body weight regulation in humans.
Case-control study comparing obese subjects from the SOS registry and a sample of the Swedish general population (body mass index (BMI) <27 kg/m2) with respect to genotype and allele frequencies of the UCP1 A-3826G polymorphism.
A total of 985 Swedish subjects including 674 obese (310 Male; 364 Female) and 311 non-obese subjects (54 Male; 257 Female) from the SOS cohorts.
DNA was extracted from total blood and genotyped by PCR-RFLP. Obesity-related phenotypes include weight history for SOS obese cohort and current weight, BMI, waist circumference and waist to hip ratio (WHR) for obese and normal weight subjects.
No significant difference in the allelic frequencies between obese and non-obese subjects (0.25 vs 0.24; P = 0.67). In both genders, current weight, BMI, waist circumference, WHR and weight gain over time (either measures of maximal weight ever achieved minus weight at 20 y or current weight minus weight at 20 y) were similar in carriers and non-carriers of the UCP1 A-3826G mutation (P>0.05). Similar results were obtained when the three genotypes were compared.
In contrast to what was found in other populations, the UCP1 A-3826G sequence variation is not associated with obesity-related phenotypes and weight gain over time in subjects from the SOS cohorts.
在瑞典肥胖受试者（SOS）队列中的肥胖和非肥胖男性及女性中，研究解偶联蛋白1（UCP1）5'侧翼区域-3826 bp处A-G点突变（UCP1 A-3826G）与一些肥胖表型之间的关系。先前的研究支持了UCP1 A-3826G多态性与人类体重调节之间存在关联的假说。
病例对照研究，比较SOS登记处的肥胖受试者和瑞典普通人群样本（体重指数（BMI）<27 kg/m²）在UCP1 A-3826G多态性的基因型和等位基因频率方面的差异。
共有985名瑞典受试者，包括来自SOS队列的674名肥胖者（310名男性；364名女性）和311名非肥胖者（54名男性；257名女性）。
从全血中提取DNA，并通过PCR-RFLP进行基因分型。与肥胖相关的表型包括SOS肥胖队列的体重史，以及肥胖和正常体重受试者的当前体重、BMI、腰围和腰臀比（WHR）。
肥胖和非肥胖受试者之间的等位基因频率无显著差异（0.25对0.24；P = 0.67）。在男女两性中，UCP1 A-3826G突变携带者和非携带者的当前体重、BMI、腰围、WHR以及随时间的体重增加（以达到的最大体重减去20岁时的体重或当前体重减去20岁时的体重来衡量）相似（P>0.05）。比较三种基因型时也得到了类似结果。
与其他人群的研究结果相反，在SOS队列受试者中，UCP1 A-3826G序列变异与肥胖相关表型及随时间的体重增加无关。
