Mansfield K G, Carville A, Hebert D, Chalifoux L, Shvetz D, Lin K C, Tzipori S, Lackner A A
New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA.
J Clin Microbiol. 1998 Aug;36(8):2336-8. doi: 10.1128/JCM.36.8.2336-2338.1998.
Enterocytozoon bieneusi is the most common microsporidian parasite recognized in human patients with AIDS. Recently, we identified a virtually identical organism causing a spontaneous infection associated with hepatobiliary and intestinal disease in simian immunodeficiency virus (SIV)-infected macaques. To examine the natural history of the infection, we examined captive rhesus macaques for E. bieneusi by PCR, in situ hybridization, and cytochemical techniques. PCR performed on fecal DNA detected enterocytozoon infection in 22 (16.7%) of 131 normal rhesus macaques (Macaca mulatta), compared to 18 (33.8%) of 53 rhesus macaques experimentally inoculated with SIV. In normal rhesus macaques, persistence of infection was demonstrated for up to 262 days and was usually not associated with clinical signs. In six of seven normal rhesus animals, E. bieneusi was detected by PCR in bile obtained through percutaneous cholecystocentesis but not by in situ hybridization performed on endoscopic biopsies of duodenum and proximal jejunum.
肠内微孢子虫是在艾滋病患者中发现的最常见的微孢子虫寄生虫。最近，我们在感染猿猴免疫缺陷病毒（SIV）的猕猴中鉴定出一种几乎相同的病原体，它可引发与肝胆和肠道疾病相关的自发性感染。为了研究这种感染的自然史，我们通过聚合酶链反应（PCR）、原位杂交和细胞化学技术，对圈养的恒河猴进行了肠内微孢子虫检测。对粪便DNA进行的PCR检测发现，131只正常恒河猴（猕猴）中有22只（16.7%）感染了肠内微孢子虫，而在53只经实验接种SIV的恒河猴中，这一比例为18只（33.8%）。在正常恒河猴中，感染可持续长达262天，且通常与临床症状无关。在7只正常恒河猴中的6只中，通过经皮胆囊穿刺获得的胆汁进行PCR检测发现了肠内微孢子虫，但对十二指肠和空肠近端进行内镜活检的原位杂交检测未发现该病原体。
