Alcalá L, García-Garrote F, Cercenado E, Peláez T, Ramos G, Bouza E
Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
J Clin Microbiol. 1998 Aug;36(8):2386-8. doi: 10.1128/JCM.36.8.2386-2388.1998.
Susceptibility testing of Eikenella corrodens is usually performed by a Mueller-Hinton sheep blood agar dilution (AD) method. However, this method is impractical for testing only a few strains. We compared AD with the broth microdilution method using Haemophilus test medium (HTM) in order to determine the susceptibility of 36 clinical isolates of E. corrodens to eight antimicrobial agents. MICs obtained by the HTM method yielded 95.5 and 84% agreement (within 2 and 1 log2 dilutions, respectively) with those obtained by AD. The HTM method with incubation in CO2 for 48 h was highly reproducible and constitutes an easy alternative for antimicrobial susceptibility testing of E. corrodens.
腐蚀艾肯菌的药敏试验通常采用穆勒-欣顿羊血琼脂稀释法(AD法)进行。然而,这种方法对于仅检测少数菌株来说并不实用。我们将AD法与使用嗜血杆菌测试培养基(HTM)的肉汤微量稀释法进行了比较,以确定36株临床分离的腐蚀艾肯菌对8种抗菌药物的敏感性。通过HTM法获得的最低抑菌浓度(MIC)与通过AD法获得的MIC分别在2倍和1个对数2稀释范围内的一致性为95.5%和84%。在二氧化碳环境中孵育48小时的HTM法具有高度可重复性,是腐蚀艾肯菌抗菌药敏试验的一种简便替代方法。