Saito M, Guidotti A, Berg M J, Marks N
Nathan S. Kline Institute of Psychiatric Research, Center for Neurochemistry, Orangeburg, New York 10962, USA.
Ann N Y Acad Sci. 1998 Jun 19;845:253-62. doi: 10.1111/j.1749-6632.1998.tb09678.x.
LIGA20 (a semisynthetic GM1 with n-dichloroacetyl sphingosine) having antinecrotic properties was evaluated as an antiapoptotic agent using cerebellar granule cells as a neuronal model. Neurons exposed to medium containing 5 mM K+ in absence of serum (apoptotic medium) for up to 22 h resulted in 65% cell death, accompanied by oligonucleosomal laddering, and a 15-fold-increase in caspase-3 activity. The relationships between times required for enzyme activation and cell death suggests a linkage between these two events. Pretreatment with 10 microM LIGA20 for 10 min significantly attenuated the aforementioned apoptotic changes. LIGA20 also attenuated apoptosis induced by Pb2+, ethanol, and C6-ceramide. Data show that LIGA20 is a versatile neuroprotective agent protecting cells from multiple pathways of cell death.
具有抗坏死特性的LIGA20（一种带有n - 二氯乙酰鞘氨醇的半合成GM1），以小脑颗粒细胞作为神经元模型，被评估为一种抗凋亡剂。在无血清（凋亡培养基）的情况下，将神经元暴露于含有5 mM K+的培养基中长达22小时，导致65%的细胞死亡，伴有寡核小体梯状条带形成，且caspase - 3活性增加了15倍。酶激活所需时间与细胞死亡之间的关系表明这两个事件之间存在联系。用10 microM LIGA20预处理10分钟可显著减轻上述凋亡变化。LIGA20还减轻了由Pb2 +、乙醇和C6 - 神经酰胺诱导的凋亡。数据表明，LIGA20是一种多功能神经保护剂，可保护细胞免受多种细胞死亡途径的影响。
