反刍动物考德里氏体天然蛋白和重组蛋白的细胞反应分析

Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium.

作者信息

Totté P, McKeever D, Jongejan F, Barbet A, Mahan S M, Mwangi D, Bensaid A

机构信息

CIRAD-EMVT, Point-à-Pitre, Gaudeloupe, French West Indies.

出版信息

Ann N Y Acad Sci. 1998 Jun 29;849:155-60. doi: 10.1111/j.1749-6632.1998.tb11045.x.

DOI:10.1111/j.1749-6632.1998.tb11045.x

PMID:9668461

Abstract

The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were more than 90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only one cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.

摘要

对用灭活考德里氏体免疫的牛所采集的外周血单核细胞（PBMC），在体外对MAP1、MAP1 - B、MAP2以及考德里氏体裂解物的反应特性进行了研究。CD4 + T淋巴细胞对考德里氏体裂解物反应强烈，而B细胞和γδ T细胞则对重组蛋白产生增殖反应。使用考德里氏体裂解物从PBMC衍生的短期细胞系中，超过90%为CD4 + ，且对任何重组蛋白均无反应。用MAP1、MAP1 - B和MAP2对PBMC进行再次刺激很困难，培养物通常在2至3周后停止增殖。仅用MAP1可生成一个细胞系，其由85%的γδ T细胞组成。本研究表明，在用灭活考德里氏体免疫的动物中，除血清学上免疫显性的MAP1和MAP2之外的蛋白质对T淋巴细胞具有强烈的免疫原性。