Mondry R, Martinez D, Camus E, Liebisch A, Katz J B, Dewald R, van Vliet A H, Jongejan F
CIRAD-EMVT, Pointe-à-Pitre, Guadeloupe, French West Indies.
Ann N Y Acad Sci. 1998 Jun 29;849:262-72. doi: 10.1111/j.1749-6632.1998.tb11058.x.
Serological tests for Cowdria ruminantium infection have been hampered by low specificity. Here, an indirect ELISA based on purified antigen, a competitive ELISA using a recombinant major antigenic protein (MAP-1) and an indirect ELISA based on the MAP-1B region of the recombinant MAP-1 were compared. The tests were validated using 3000 sera of ruminants from 14 islands of the Lesser Antilles as well as sequential serum samples from 10 cattle, 17 goats and 10 sheep vaccinated with inactivated C. ruminantium in ISA 50 adjuvant and from 14 goats infected with a virulent culture supernatant. All tests detected significantly higher percentages of positives on Antigua, Guadeloupe and Marie-Galante, where C. ruminantium had been isolated before. Overall specificity calculated with sera from the other 11 heartwater-free islands was 98.1%, 98.5%, and 99.4% for the ELISA based on crude antigen, recombinant MAP-1 and MAP-1B, respectively. Sensitivities observed with sequential serum samples were similar for all tests. Tests based on recombinant antigens, especially the MAP-1B, showed improved specificity, suggesting their use for epidemiological studies in regions where the distribution of cowdriosis is unknown. In addition, the competitive ELISA is useful for studies in wildlife for which species-specific conjugates do not exist.
反刍兽考德里氏体感染的血清学检测一直受到低特异性的困扰。在此，对基于纯化抗原的间接ELISA、使用重组主要抗原蛋白（MAP-1）的竞争ELISA以及基于重组MAP-1的MAP-1B区域的间接ELISA进行了比较。使用来自小安的列斯群岛14个岛屿的3000份反刍动物血清以及来自10头牛、17只山羊和10只绵羊的连续血清样本对这些检测方法进行了验证，这些动物用ISA 50佐剂中的灭活反刍兽考德里氏体进行了免疫接种，还有来自14只感染了强毒培养上清液的山羊的血清样本。所有检测方法在之前已分离出反刍兽考德里氏体的安提瓜岛、瓜德罗普岛和玛丽-加朗特岛上检测到的阳性百分比显著更高。用来自其他11个无牛心水病岛屿的血清计算得出，基于粗抗原、重组MAP-1和MAP-1B的ELISA的总体特异性分别为98.1%、98.5%和99.4%。所有检测方法对连续血清样本观察到的敏感性相似。基于重组抗原的检测方法，尤其是MAP-1B，显示出更高的特异性，表明它们可用于牛心水病分布未知地区的流行病学研究。此外，竞争ELISA对于不存在物种特异性结合物的野生动物研究很有用。
